Protein Info for mRNA_1908 in Rhodosporidium toruloides IFO0880
Name: 10276
Annotation: K01426 E3.5.1.4, amiE amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (564 amino acids)
>mRNA_1908 K01426 E3.5.1.4, amiE amidase (Rhodosporidium toruloides IFO0880) MAITTSPYHQTLPHLTKDNWRDHAADKRARQAALIPKEWRLSPETLDATGDDVTGVPKTC GILSERELEITELDEVEELAKRIAKKTYTAVEVATAFSKRAAIAQQLTNCLTEIFFSEAI QQATKLDEILEKTGKTVGPLHGVPVSLKDQFDVAGTESTMGYCSYLGRISTYDSALVRLL RDAGAVFHCRTNIPQTLMIGDVFNHLFGRCCNPHNRKLIPGGSSGGEGALIAMKGSIVGV GTDVGGSVRIPSAMCGLHTIRPTTRRLPYAGATTSLIGQKAVVCVAGPMARSLSSCAYFL RAVLNLNPANYDPTPLPFPFNDAAYTCTAKRVKLAFGIIRTDHNVTPTPPIKRALDLAAQ KLVNAGHEVIEFDSVLLKDAFQLANDIFGADGGEDFKRALDPIGEPLIPGISPTQNSLKT VSEFWQLNYAKELVQQGFLAQWLATAEQTSTGRPIDGLLCPPAPVTAVLPGGNRHAGYTG MFNLLDVPVLCFPVTKVEPAVDSADPYFKPLNEEDRRFHDEYDPQLTAGMPVGIQLVGRR WQDEELLGLGEVVASIIGSKDWAE