Protein Info for mRNA_2245 in Rhodosporidium toruloides IFO0880

Name: 10613
Annotation: K00134 GAPDH, gapA glyceraldehyde 3-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00044: Gp_dh_N
  • amino acids 8 to 109 (102 residues), 134.3 bits, E=1.8e-43
• TIGR01534: glyceraldehyde-3-phosphate dehydrogenase, type I
  • amino acids 9 to 330 (322 residues), 443.8 bits, E=2e-137
• PF02800: Gp_dh_C
  • amino acids 161 to 318 (158 residues), 240.1 bits, E=8.3e-76

Best Hits

Swiss-Prot: 81% identical to G3P_CUTCU: Glyceraldehyde-3-phosphate dehydrogenase (GPD) from Cutaneotrichosporon curvatus

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 79% identity to uma:UM02491.1)

MetaCyc: 71% identical to glyceraldehyde-3-phosphate dehydrogenase monomer (Homo sapiens)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). [EC: 1.2.1.12]; RXN-12753 [EC: 1.2.1.12]; RXN-12754 [EC: 1.2.1.12]

Predicted SEED Role

"NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Pyridoxin (Vitamin B6) Biosynthesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.12)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• Bifidobacterium shunt (14/15 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/12 steps found)
• homolactic fermentation (10/12 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis IV (8/10 steps found)
• superpathway of glucose and xylose degradation (13/17 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/16 steps found)
• gluconeogenesis III (9/12 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• Entner-Doudoroff pathway I (6/9 steps found)
• glycerol degradation to butanol (11/16 steps found)
• NADH repair (eukaryotes) (2/4 steps found)
• NADH repair (prokaryotes) (2/4 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (7/12 steps found)
• heterolactic fermentation (11/18 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• pyridoxal 5'-phosphate biosynthesis I (3/7 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (11/19 steps found)
• superpathway of N-acetylneuraminate degradation (11/22 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.12

Use Curated BLAST to search for 1.2.1.12

Protein Sequence (345 amino acids)

>mRNA_2245 K00134 GAPDH, gapA glyceraldehyde 3-phosphate dehydrogenase (Rhodosporidium toruloides IFO0880)
MSAGKGSVNVGINGFGRIGRIVLRNAIEHGDARVVAINDPFIDLEYMVYMFKYDSTHGRF
KGTIEAKDGKLVVDGHAIDVYNEKDPASIPWSKSGADYVVESTGVFTTKEKAGLHLKGGA
KKVVISAPSADAPMYVCGVNLDKYNPADSVISNASCTTNCLAPLAKVINDKFGIVEGLMT
TVHATTATQKTVDGPSAKDWRGGRAAAANIIPSSTGAAKAVGKVIPELNGKLTGMAFRVP
TTDVSVVDLTVRLEKGASYDQIKQTIREAANGELKGILEYTEDALVSTDFVGHTASSIFD
ASAGIALNDNFVKLVSWYDNEYGYSRRVVDLLVFVAKQDAAGGKL