Protein Info for mRNA_2473 in Rhodosporidium toruloides IFO0880

Name: 10841
Annotation: K01610 E4.1.1.49, pckA phosphoenolpyruvate carboxykinase (ATP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 61% identical to PCKA_EMENI: Phosphoenolpyruvate carboxykinase (ATP) (acuF) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 75% identity to uma:UM05130.1)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
gluconeogenesis I (11/13 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (6/14 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (662 amino acids)

>mRNA_2473 K01610 E4.1.1.49, pckA phosphoenolpyruvate carboxykinase (ATP) (Rhodosporidium toruloides IFO0880)
MLAAKHANPRLVVVASASSSSGRLHVRDLATTPGDFAPAATGAQRHHSTLAGPTTARESS
RRREEGDKERKVVQGAVGAPTASPWLCNSPQMTRTVQKSLWAAASQWDAVLRPGKVRNAP
DDSSQPTYGLLGKDLAMFSEAGFDMDKIHLKRNAPVAQLYEDAIKYEGAVIAASGALINF
SGKKTGRSPKDKRIVYEETSKDDVWWGAINIQMDEHTFEINRERAIDYLNTRDNVYVFDG
FAGWDPKYRIKVRVIASRAYHALFMHNMLIRPTAKELEEFGTPDFTIYNAGQFPANRFTA
GMTSQTSVEVNFKRREMVILGTEYAGEMKKGIFSVMHYLQPVKFGNLSLHSSANQGPDGD
VSLFFGLSGTGKTTLSADPRRALIGDDEHVWSDNGVFNIEGGCYAKCVGLDAEKEPAIFN
AIRFGSILENVTYDYATREPNYEDTGITENTRCAYPIEYIDNAKIPCMANEQPKNVIYLT
CDAFGVLPPVSLLDSNQAQYWFLSGYTSKTPGTEDGIAEPTPTFSTCFGAPFIILHPSRY
ASMLAKKMEAAGTRCWLINTGWVGGAFGTGKRCPLKYTRAIVDAVHSGELNNADFKETPV
FGLKVPTKIEGIPDDVLNPEAAWSDKKAYQQTLEKLAGMFNKAFERYSTECSPEVVAAGP
KI