Protein Info for mRNA_2855 in Rhodosporidium toruloides IFO0880

Name: 11223
Annotation: K10747 LIG1 DNA ligase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 872 PF04675: DNA_ligase_A_N" amino acids 247 to 423 (177 residues), 169.2 bits, see alignment E=1.7e-53 TIGR00574: DNA ligase I, ATP-dependent (dnl1)" amino acids 305 to 849 (545 residues), 540 bits, see alignment E=3.2e-166 PF01068: DNA_ligase_A_M" amino acids 493 to 698 (206 residues), 219.3 bits, see alignment E=6.8e-69 PF04679: DNA_ligase_A_C" amino acids 723 to 833 (111 residues), 84.2 bits, see alignment E=1.2e-27

Best Hits

KEGG orthology group: K10747, DNA ligase 1 [EC: 6.5.1.1] (inferred from 60% identity to cne:CNI04170)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (872 amino acids)

>mRNA_2855 K10747 LIG1 DNA ligase 1 (Rhodosporidium toruloides IFO0880)
MAKGEQPSGERAPDQGGLNAFLGKPDSRPHQKTLNFFSKDQRDRQTAPSPAVEPKAEQKD
VEMSPAADEANERKEKKRTATEAAEEEGGADKDDKASPSKKAKTSNGDAVAEKGASPSKD
KDAASPVASSSSAAKASTSASPSTSKAKADDSDKPPVSSFFAPRSTTAPKKASSDDKGKA
KAKVQDDEEEREGRDASEAPSGEGEVSEEEEEDEEDEKAAVKFADIFTKSSKPDKAQTSW
KSGEPVPYAALTATFSKIEATTKRLEISAYLTRFLIDVIEKTPEDLLKTVYLCINRLAPE
YEPIELGIGESLLMKAIGESCGRTLAQVKAEYKKIGDLGEVAFNSRTRQKTLFKSKPLTV
KGVFDELKKVAKTSGKDSQGRKVGLIKSLLAKCEGEETKFLIRSLEGKLRIGLAEKTVLV
SLAHAAVTAEHNKSGKKWSRERLSAQLEEGAEILKSVFSEIPSYDLVVPALLDKGMSHLQ
EACSLTPGIPLKPMLAKPTKAITEVLDRFEGKQFTCEYKYDGERAQIHYLEDGSIKVFSR
NSEDMTVKYPEFVTQLPKCIKPNTKSFVIDAEAVAWDTEEKRLLEFQKLSTRKRKDVKAE
DIKVKVHLFAFDLLYLNGESLLEKNLRERRELLRDHLQPVEGEFAFAQSDDASTVEEIQT
FLDQSIKDGCEGLMVKMLEGEGASYEPSRRSIHWLKLKKDYLQGVGDSFDLVVIGGDYGK
GKRTNVYGAFHLACFDAESGTYQMICKIGTGFSDEALKQHHETLKPLELAQKKAYYDIGT
AKGPDVYFEPKVVWEVLAADLSLSPIYSAAKGLCGDRGISLRFPRFIRVRDDKDPESSTE
PEQIAEAYRRQGIHTAGGGKKGKGAADDGDFW