Protein Info for mRNA_3786 in Rhodosporidium toruloides IFO0880

Name: 12154
Annotation: K00006 GPD1 glycerol-3-phosphate dehydrogenase (NAD+)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03376: glycerol-3-phosphate dehydrogenase (NAD(+))
  • amino acids 13 to 348 (336 residues), 485.3 bits, E=5.1e-150
• PF01210: NAD_Gly3P_dh_N
  • amino acids 13 to 181 (169 residues), 170.3 bits, E=3.3e-54
• PF07479: NAD_Gly3P_dh_C
  • amino acids 202 to 347 (146 residues), 154.8 bits, E=1.8e-49

Best Hits

Swiss-Prot: 56% identical to GPDA_MOUSE: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (Gpd1) from Mus musculus

KEGG orthology group: K00006, glycerol-3-phosphate dehydrogenase (NAD+) [EC: 1.1.1.8] (inferred from 62% identity to uma:UM00555.1)

MetaCyc: 55% identical to cytoplasmic glycerol-3-phosphate dehydrogenase subunit (Homo sapiens)
Glycerol-3-phosphate dehydrogenase (NAD(+)). [EC: 1.1.1.8]

Predicted SEED Role

"Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 1.1.1.94)

MetaCyc Pathways

• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• phosphatidylglycerol biosynthesis I (plastidic) (5/6 steps found)
• phosphatidylglycerol biosynthesis II (non-plastidic) (5/6 steps found)
• glycerol-3-phosphate shuttle (2/2 steps found)
• superpathway of phospholipid biosynthesis I (bacteria) (9/12 steps found)
• CDP-diacylglycerol biosynthesis III (3/5 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• 1,3-propanediol biosynthesis (engineered) (5/9 steps found)
• glucosylglycerol biosynthesis (2/5 steps found)
• superpathway of phospholipid biosynthesis II (plants) (15/28 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.8, 1.1.1.94

Use Curated BLAST to search for 1.1.1.8 or 1.1.1.94

Protein Sequence (351 amino acids)

>mRNA_3786 K00006 GPD1 glycerol-3-phosphate dehydrogenase (NAD+) (Rhodosporidium toruloides IFO0880)
MPAQTSSDGGKWKVALIGSGNWGSAIARLVGKNIKERSDEFDTEVKMWCYEEDFDGRKLT
EVINEKHENIKYLPGIDLGDNVVAEPDLIKAIEGANALIFVIPHQFIIETCKQLEGKVPS
NTQAISLVKGVDVKDGQIHIFSEVIKEHLDIECAALSGANIANEVAKEAFSETTIGCRDI
EVAKRWARLFETEKFRVAVSKDVLGVSLGGALKNVVAVAAGLIDGLQWGNNAKAAIMRIG
LLEMKRFALEFFPGTEPETFVQESAGIADLITSCLGGRNRKCAEAFVTTGKTFDQLEKEM
LNGQKLQGVQTAKELHEFLKARGKADNYPLFTFVYRVAYEGVKPDTITAHI