Protein Info for mRNA_3880 in Rhodosporidium toruloides IFO0880

Name: 12248
Annotation: K00262 E1.4.1.4, gdhA glutamate dehydrogenase (NADP+)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 50 to 165 (116 residues), 146.5 bits, E=4.2e-47
• PF00208: ELFV_dehydrog
  • amino acids 183 to 441 (259 residues), 257 bits, E=2.1e-80

Best Hits

Swiss-Prot: 67% identical to DHE4_BOTFU: NADP-specific glutamate dehydrogenase (gdhA) from Botryotinia fuckeliana

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 68% identity to pan:PODANSg8412)

MetaCyc: 59% identical to NADP-dependent glutamate dehydrogenase 2 subunit (Saccharomyces cerevisiae)
Glutamate synthase (NADPH). [EC: 1.4.1.13, 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• superpathway of L-citrulline metabolism (10/12 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (3/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.4

Protein Sequence (444 amino acids)

>mRNA_3880 K00262 E1.4.1.4, gdhA glutamate dehydrogenase (NADP+) (Rhodosporidium toruloides IFO0880)
MSSYTASKVSEPEFEQAVAEIRSTLAPLFKKRPELENAFKVLMIPERIIQFRGQCQVNNG
FRVQFSSVLGPYKGGLRLHPSVNLSILKFLGFEQIFKNALTGLHMGGGKGGSDFDPKGKS
DNEIRRFCVAFMRELSRHIGADTDVPAGDIGVGGREVGFLFGAYKQMRNEWVGVLTGKGY
DFGGSKIRPEATGYGCVYYAEEMIRVARGEDFKGKRVLISGAGNVAQFAALKVIELGGQV
LSLSDSKGALVAEGNEGLSADFINQVADLKLKGGYLKDLSAPSGFKYVEGARPWKLVKNV
DVALPSATQNELDEEDAKALVAAGLGFVAEGANMPCTLEAINVFESSRREKGNKGVWYGP
GKAANCGGVAVSGLEMAQNSARLQWTDEVVDAKLKDIMKNAFEIGLETAKEFTEGGEWPS
LVMGSNVAGFLKVVEAAKAQGDVW