Protein Info for mRNA_3934 in Rhodosporidium toruloides IFO0880

Name: 12302
Annotation: K01955 carB, CPA2 carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 88 to 1145 (1058 residues), 1463.4 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 212 to 415 (204 residues), 279.4 bits, E=5.2e-87
  • amino acids 755 to 955 (201 residues), 84.3 bits, E=2.7e-27
• PF02222: ATP-grasp
  • amino acids 221 to 367 (147 residues), 29.4 bits, E=1.7e-10
  • amino acids 764 to 911 (148 residues), 33.3 bits, E=1.1e-11
• PF07478: Dala_Dala_lig_C
  • amino acids 240 to 383 (144 residues), 26 bits, E=2e-09
• PF02787: CPSase_L_D3
  • amino acids 501 to 629 (129 residues), 139.2 bits, E=2.7e-44

Best Hits

Swiss-Prot: 64% identical to CARB_ASHGO: Carbamoyl-phosphate synthase arginine-specific large chain (CPA2) from Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 69% identity to act:ACLA_069100)

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (38/46 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (15/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• urea cycle (5/5 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine biosynthesis IV (archaea) (6/9 steps found)
• L-glutamate and L-glutamine biosynthesis (3/6 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1180 amino acids)

>mRNA_3934 K01955 carB, CPA2 carbamoyl-phosphate synthase large subunit (Rhodosporidium toruloides IFO0880)
MLRTFSSRIRTAPSTRPLRAFHTTPRRSFVVDLTRRASIPDPSIAPTANEDLVDISSQFK
HIQTKDGTSAASDLARRLSQAVLPKLERPDVKKVMIVGSGGLSIGQAGEFDYSGAQAIKA
MKESNIECILLNPNIATIQTSHQMASEVYFLPVTAEYVAYVLEQERPDGILLTFGGQSAL
NVGVQLEKMGVFERLGVKVLGTQIRTLEVSEDRDLFVKALNEIGIPAATSTAVSTVKDAL
EAAERIGYPVILRSAFSLGGLGSGFAENEEELRDLSAKSLSLSPQVLIEKSLKGWKELEY
EVVRDAADNVIICCNMENFDPLGIHTGDSIVIAPSQTLSDDEYHMLRSAAIKIVRHVGVV
GECNVQYALNPNKREYMVIEMNARLSRSSALASKATGYPLAYTAAKIALGHTLPELPNAV
TKTTTACFEPALDYLVTKIPKWDLAKFQHVNREVGSSMKSVGEVMAIGRTFEESLQKAIR
QVDPRWTGFEAYWKPEDLEHALTVPTDRRLFAIAHAMLNLNYTVDMIHDLTKIDKWFLYK
LDNIVQVHHQFRKTKSVAEVDEDLMRKAKRMGFSDKQIADLIVRDGGEATDEYAVRAHRK
SLGVTPFVKRIDTLAAEYPAHTNYLYTTYNASSHDIEFDDKGTMVLGSGVYRIGSSVEFD
WCAVSCVRKVREMGKKTVMINYNPETVSTDFDEADRLYFEELGWERVMDIYELEGSEGVV
VSVGGQLPQNIALRLKQTGINVLGTDPESIDNAEDRHKFSAILDKSGVDQPAWVEATTVE
SAKEFAKKVGYPVLVRPSYVLSGAAMNVVWDESTLEHSLTAAAEVSSEHPTVITKFIDGA
QEIDVDAVAHKGELLIHAVSEHVENAGVHSGDATLVLPPFSLADDDMERLKVIAQKVAKA
FDISGPFNMQIIRKATPGEETTLKVIECNLRASRSFPFVSKVLGVNFIDYATAAIVGENV
PAPIDVMKEKRDYVAIKVPQFSWTRLAGADPYLGVEMASTGEVAAFGTDLAEAYWASLAS
TNGFRVPQANSGVLLGGDVNCPQLATVAKGLSELGFKLYCSSSVVEEHLNALPHVKAEKI
FFPLKDKRKLREVFDEYEIQSVINLAKARGKDTVDEDYVARRNAVDFGVPLINNPLLAEL
YVQALGRKMKKGEVGKANNYEEGRIPSEVQPWSQWIGSHA