Protein Info for mRNA_4722 in Rhodosporidium toruloides IFO0880

Name: 13090
Annotation: K01027 OXCT 3-oxoacid CoA-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 TIGR02429: 3-oxoacid CoA-transferase, A subunit" amino acids 31 to 266 (236 residues), 226.3 bits, see alignment E=2.9e-71 PF01144: CoA_trans" amino acids 37 to 265 (229 residues), 214.4 bits, see alignment E=7e-68 amino acids 302 to 501 (200 residues), 129.9 bits, see alignment E=4.8e-42 TIGR02428: 3-oxoacid CoA-transferase, B subunit" amino acids 300 to 508 (209 residues), 291.3 bits, see alignment E=3.3e-91

Best Hits

Swiss-Prot: 57% identical to SCOT1_HUMAN: Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (OXCT1) from Homo sapiens

KEGG orthology group: K01027, 3-oxoacid CoA-transferase [EC: 2.8.3.5] (inferred from 62% identity to cpw:CPC735_047100)

MetaCyc: 57% identical to succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial (Homo sapiens)
3-oxoacid CoA-transferase. [EC: 2.8.3.5]

Predicted SEED Role

"Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5)" in subsystem Catechol branch of beta-ketoadipate pathway or Leucine Degradation and HMG-CoA Metabolism or Protocatechuate branch of beta-ketoadipate pathway or Serine-glyoxylate cycle (EC 2.8.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>mRNA_4722 K01027 OXCT 3-oxoacid CoA-transferase (Rhodosporidium toruloides IFO0880)
MLVSTSRRFLTRLPTRAFSTSSARLDVKKTVSKIYPSAEEAVKDVKGDSIVLSGGFGLCG
TADTLIGALAKRPEVKNLTCVSNNAGVGKMGLGALLHSGQISKMISSYLGANKHFESLYL
TGQVSLELTPQGTLAERCRAGGAGIPAFYTPAGYGTAVQTGEIPIKYKEGGKEVEVPGKP
RETREFNGKGYLMEEAIKGDYALVKVWKADEYGNCVFRYAAQNFSGAMARSAKMTIVEAE
EIVPVGSLDPNNVHLPGIFVNRVVQSTAPKQIEIEVLREEASAGGSASEAAMGKDEARLR
RERIVKRAAKELKEGMYVNLGIGMPMLAPSFLEPGTVIHMQSENGILGMGPYPTKSEVDP
DIINAGKETVTLLPGASTFDSAESFSMIRGGHVDVSMLGAMQVSASGDIANYMIPGKMVK
GMGGAMDLVSNPEQTKVVVVMDHVAKGGKHKILDECSLPLTGVNCVSTIITDLCVFQVDR
QAKKLILTELAAGVTEEDVRKATGAKYEVSPDLVTMDA