Protein Info for mRNA_5262 in Rhodosporidium toruloides IFO0880

Name: 13630
Annotation: K00161 PDHA, pdhA pyruvate dehydrogenase E1 component alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03182: pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit
  • amino acids 68 to 381 (314 residues), 421.6 bits, E=7.9e-131
• PF00676: E1_dh
  • amino acids 76 to 371 (296 residues), 381 bits, E=1.7e-118

Best Hits

Swiss-Prot: 58% identical to ODPA_YEAST: Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial (PDA1) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 70% identity to cci:CC1G_09082)

Predicted SEED Role

"Pyruvate dehydrogenase E1 component alpha subunit (EC 1.2.4.1)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• TCA cycle III (animals) (10/10 steps found)
• TCA cycle II (plants and fungi) (9/9 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (2/3 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (2/4 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (16/26 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (4/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (8/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.1

Use Curated BLAST to search for 1.2.4.1

Protein Sequence (429 amino acids)

>mRNA_5262 K00161 PDHA, pdhA pyruvate dehydrogenase E1 component alpha subunit (Rhodosporidium toruloides IFO0880)
MLRTTLAKAIRPAVQARTPALARSVQTTADSARAIDTSGDKVKVQLHEEYFKAYRCDTPS
LEMEVPKNQLVQVYKDMVEMRRMEMACDQLYKAKMIRGFCHLAIGQEAVSVGMHHAIKPD
DKIITAYRCHPFAALRGGSVKGVIAELLGRVDGMSHGKGGSMHIFTPTFFGGNGIVGAQV
PLGAGIAFAQQYLGQDNEHATFAMYGDGASNQGQVFEAYNMAKLWNLPCVFVCENNLYGM
GTSAARSSSNTKYYQRGDLIPGIQVNGMDVLAVMKAVRFAKEWTTSGKGPLLLEMVTYRY
GGHSMSDPGTTYRTREEIQHMRSSNDPITGLKNNLLEWNVIEESALKKIDKEVRATVEKA
VEEAKKSPEPSQEKDMWTDIYYKGTNPMWLRGREREEVHHYQDSDLLPNGPKQFGAGGNT
QMESMTSTV