Protein Info for mRNA_5633 in Rhodosporidium toruloides IFO0880

Name: 14001
Annotation: K01679 E4.2.1.2B, fumC fumarate hydratase, class II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00979: fumarate hydratase, class II
  • amino acids 14 to 473 (460 residues), 773.1 bits, E=4.7e-237
• PF00206: Lyase_1
  • amino acids 22 to 354 (333 residues), 387.3 bits, E=6.3e-120
• PF10415: FumaraseC_C
  • amino acids 420 to 473 (54 residues), 79.5 bits, 2.1e-26

Best Hits

Swiss-Prot: 80% identical to FUMH_RHIOR: Fumarate hydratase, mitochondrial (FUMR) from Rhizopus oryzae

KEGG orthology group: K01679, fumarate hydratase, class II [EC: 4.2.1.2] (inferred from 80% identity to ppl:POSPLDRAFT_86820)

MetaCyc: 72% identical to fumarase subunit (Ascaris suum)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• TCA cycle III (animals) (10/10 steps found)
• TCA cycle II (plants and fungi) (9/9 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• mixed acid fermentation (9/16 steps found)
• pyruvate fermentation to propanoate I (2/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (4/10 steps found)
• reductive TCA cycle II (5/12 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (8/17 steps found)
• methylaspartate cycle (9/19 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (4/13 steps found)
• L-glutamate degradation VIII (to propanoate) (2/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (26/57 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (477 amino acids)

>mRNA_5633 K01679 E4.2.1.2B, fumC fumarate hydratase, class II (Rhodosporidium toruloides IFO0880)
MSIAPTLQPGQDKFRVERDTFGELQVPADKYYGAQTQRSVQNFDIGGPSERMPPPLIKAF
AVLKKAAAEVNMTYGLDSKVGEAIKQAADEVIAGKIGQDHFPLVVFQTGSGTQTNMNVNE
VISNRAIEILGGELGSKKPVHPNDHVNMSQSSNDTFPTAMHVSAVVEISNSLIPALEELK
AALYEKQQSFEKIIKIGRTHLQDATPLTLGQEFSGYVQQVENSIARVKDVLPRLSKLAQG
GTAVGTGLNTRKGFDSKVAAEISKITGLSFETAPNKFEALAAHDAIVEASGALNTVACSF
NKIANDIRYLGSGPRCGLGELALPENEPGSSIMPGKVNPTQCEALTMVAAQVVGNNMAIT
VGGLQGQFELNVFKPVMIKNLLQSIRILSDGARSFTKNCVVGIQANEERIAKLLNESLML
VTCLNSRIGYDNAAKCAKKAHKEGTTLKEATLSLGLLDSEEFDRLVRPELMVGPSDA