Protein Info for mRNA_5741 in Rhodosporidium toruloides IFO0880

Name: 14109
Annotation: K00121 frmA, ADH5, adhC S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 72% identical to FADH_CANMA: S-(hydroxymethyl)glutathione dehydrogenase (FDH1) from Candida maltosa

KEGG orthology group: K00121, S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase [EC: 1.1.1.1 1.1.1.284] (inferred from 76% identity to cne:CNM01690)

MetaCyc: 68% identical to S-(hydroxymethyl)glutathione dehydrogenase subunit (Saccharomyces cerevisiae)
S-(hydroxymethyl)glutathione dehydrogenase. [EC: 1.1.1.284]; 1.1.1.284 [EC: 1.1.1.284]; Indole-3-acetaldehyde reductase (NADH). [EC: 1.1.1.284, 1.1.1.190]; Alcohol dehydrogenase. [EC: 1.1.1.284, 1.1.1.190, 1.1.1.1]; 1.1.1.1 [EC: 1.1.1.284, 1.1.1.190, 1.1.1.1]; 1.1.1.1 [EC: 1.1.1.284, 1.1.1.190, 1.1.1.1]; 1.1.1.1 [EC: 1.1.1.284, 1.1.1.190, 1.1.1.1]; 1.1.1.1 [EC: 1.1.1.284, 1.1.1.190, 1.1.1.1]

Predicted SEED Role

"S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 1.1.1.284)

MetaCyc Pathways

3-methylbutanol biosynthesis (engineered) (6/7 steps found)
ethanol degradation II (3/3 steps found)
formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
pyruvate fermentation to ethanol II (2/2 steps found)
pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
superpathway of anaerobic sucrose degradation (14/19 steps found)
acetaldehyde biosynthesis I (1/1 steps found)
L-tryptophan degradation VIII (to tryptophol) (3/4 steps found)
phytol degradation (3/4 steps found)
salidroside biosynthesis (3/4 steps found)
L-isoleucine degradation II (2/3 steps found)
L-leucine degradation III (2/3 steps found)
L-methionine degradation III (2/3 steps found)
L-valine degradation II (2/3 steps found)
ethanol degradation I (1/2 steps found)
phenylethanol biosynthesis (3/5 steps found)
protein S-nitrosylation and denitrosylation (3/5 steps found)
butanol and isobutanol biosynthesis (engineered) (5/8 steps found)
L-phenylalanine degradation III (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
pyruvate fermentation to ethanol I (1/3 steps found)
pyruvate fermentation to ethanol III (1/3 steps found)
(S)-propane-1,2-diol degradation (2/5 steps found)
acetylene degradation (anaerobic) (2/5 steps found)
ethanolamine utilization (2/5 steps found)
heterolactic fermentation (11/18 steps found)
serotonin degradation (3/7 steps found)
mixed acid fermentation (9/16 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (11/19 steps found)
superpathway of fermentation (Chlamydomonas reinhardtii) (3/9 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
superpathway of N-acetylneuraminate degradation (11/22 steps found)
noradrenaline and adrenaline degradation (4/13 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
L-tryptophan degradation V (side chain pathway) (1/13 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1, 1.1.1.284

Use Curated BLAST to search for 1.1.1.1 or 1.1.1.190 or 1.1.1.284

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (381 amino acids)

>mRNA_5741 K00121 frmA, ADH5, adhC S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase (Rhodosporidium toruloides IFO0880)
MSDTQGKVITCKAAVAWEAGKPVSIEEITVDPPKEGEVRIKVLYTALCHTDLFTLSGDDP
EGAFPVVLGHEGGGIVESVGEGVTDVKVGDHVVPLYTAECRECKFCKSGKTNLCGAVRAT
QGQGKMPDGTRRFKCKGKELLHFMGCSTFSQYTVVSKYSVVAITDKAPLDKACLLGCGLT
TGYGAATKTANVQEGDNVAVFGLGAVGLAVIQGCKVRKAKQIIAIDTNPGKKDWAMSLGA
TDFINPLDLGKDKSIVDVLVEKTDGGCEHTFDCTGNVQVMRQALEACHKGWGVSTIIGVA
AAGKEISTRPFQLVTGRKWQGSAFGGVKGRTELPGLVDDYLAGKLTVDDYVTHRTSLDKI
DQGFGFMHEGQCIRCVVDMWA