Protein Info for mRNA_5794 in Rhodosporidium toruloides IFO0880

Name: 14162
Annotation: K01637 E4.1.3.1, aceA isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00463: ICL
  • amino acids 73 to 600 (528 residues), 625.7 bits, E=6.2e-192
• TIGR01346: isocitrate lyase
  • amino acids 74 to 600 (527 residues), 595.6 bits, E=3.6e-183
• PF13714: PEP_mutase
  • amino acids 175 to 305 (131 residues), 37.3 bits, E=2.2e-13

Best Hits

Swiss-Prot: 57% identical to ACEB_YARLI: 2-methylisocitrate lyase, mitochondrial (YALI0F31999g) from Yarrowia lipolytica (strain CLIB 122 / E 150)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 62% identity to cci:CC1G_06620)

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1), group II (plants and fungi)" (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.1

Use Curated BLAST to search for 4.1.3.1

Protein Sequence (600 amino acids)

>mRNA_5794 K01637 E4.1.3.1, aceA isocitrate lyase (Rhodosporidium toruloides IFO0880)
MLRTAAARSARSLRPSSLHSIAPRIRTSAASLSRAMSSSAVRASAGAAQSQQPLFYLDPP
SAEQEEANFRTQVDDISKWFSSPRWTKTKRPYSAELVATKRGTQPISPLHPSNVSAKKLW
AQLERASEKGVPLHTMGAIDPVQMTQQAPYLDVLYVSGWAASSVLTTGFNEVGPDLADYP
YTTVPNQVQRLRKAQELHDRKMWDERRCGNKDKQPWIDYLRPIVADGDTGHGGLSSVMKL
AKAFGEAGVSAVHFEDQLHGGKKCGHQAGKVLVPISEHVSRLVAARLQWDIMGLETLLIA
RTDAESGKLLSSTADARDHEFVLGVETHNGPKKALADEIMHASLAGKSGAEIDQLEADWM
SGVKLVTFDEAVENHLKRTLSASDASSKFETYEQRVAGANLSNREARAVAKEIVGEEIQW
DWDLPRTKEGYYHYRGGMAAAMKRTLAFSDYADLLWLETKAPDLKQAQTFAGTIREKNPG
KWLVYNLSPSFNWGAHGFSNEQLKSFVWDLGKAGFVIQLISLAGLHSTAVTTAELAKRFK
TDGMLAYVELIQQKEKDLKVDVFTHQKWSGASYSDRVLNAVAAGSSATQASGKDSTEHTF