Protein Info for mRNA_5884 in Rhodosporidium toruloides IFO0880

Name: 14252
Annotation: K13830 ARO1 pentafunctional AROM polypeptide

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01357: 3-dehydroquinate synthase
  • amino acids 29 to 389 (361 residues), 361.4 bits, E=1e-111
• PF01761: DHQ_synthase
  • amino acids 80 to 367 (288 residues), 327.6 bits, E=2e-101
• PF13685: Fe-ADH_2
  • amino acids 85 to 301 (217 residues), 31 bits, E=8.8e-11
• PF00275: EPSP_synthase
  • amino acids 411 to 863 (453 residues), 387.5 bits, E=2.8e-119
• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 417 to 865 (449 residues), 403.9 bits, E=1.5e-124
• PF01202: SKI
  • amino acids 903 to 1045 (143 residues), 87.1 bits, E=6e-28
• TIGR01093: 3-dehydroquinate dehydratase, type I
  • amino acids 1106 to 1334 (229 residues), 168.9 bits, E=3.7e-53
• PF01487: DHquinase_I
  • amino acids 1116 to 1334 (219 residues), 166.3 bits, E=5.7e-52
• TIGR01809: shikimate-5-dehydrogenase
  • amino acids 1341 to 1627 (287 residues), 234.6 bits, E=2.5e-73
• PF08501: Shikimate_dh_N
  • amino acids 1349 to 1430 (82 residues), 89.9 bits, 4.3e-29
• PF01488: Shikimate_DH
  • amino acids 1465 to 1538 (74 residues), 29.5 bits, 2.9e-10
• PF18317: SDH_C
  • amino acids 1598 to 1628 (31 residues), 38.1 bits, see alignment (E = 4.2e-13)

Predicted SEED Role

"3-dehydroquinate synthase (EC 4.2.3.4) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) / Shikimate kinase I (EC 2.7.1.71) / 3-dehydroquinate dehydratase I (EC 4.2.1.10) / Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Quinate degradation or Type IV pilus or Benzoate transport and degradation cluster (EC 1.1.1.25, EC 2.5.1.19, EC 2.7.1.71, EC 4.2.1.10, EC 4.2.3.4)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (16/18 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• superpathway of L-tryptophan biosynthesis (12/13 steps found)
• chorismate biosynthesis I (7/7 steps found)
• superpathway of L-phenylalanine biosynthesis (9/10 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• 3-dehydroquinate biosynthesis I (2/2 steps found)
• gallate biosynthesis (2/3 steps found)
• quinate degradation I (2/3 steps found)
• quinate degradation II (2/3 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (28/59 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (4/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (5/42 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.25, 4.2.1.10

Use Curated BLAST to search for 1.1.1.25 or 2.5.1.19 or 2.7.1.71 or 4.2.1.10 or 4.2.3.4

Protein Sequence (1635 amino acids)

>mRNA_5884 K13830 ARO1 pentafunctional AROM polypeptide (Rhodosporidium toruloides IFO0880)
MARTETAVDKMSILGSESIHCGFHLIPYIVETVLSTLQSSTYVLFTDTHLAPLYLSSFSS
AFEEAIDKLQIETRPRFITHVIAPGEQSKSRETKAELEDFLLSHRCTRDTVVLALGGGVV
GDLIGFVAATFMRGVRYCQIPTTLLAMVDSSVGGKTAIDTPLGKNLIGAFHQPSYIFIDA
SFLESLPRREFVNGMAEVIKTAAIWDEAEFAKLESGVADIHAAVSGSSTRDTFAGRTLET
RSAAQSLLLSVIRASIATKAHIVTVDEKETGLRNLVNFGHTIGHAIEAVVTPDVLHGECV
AVGMVLEAEVARSMGALGNAAVGRLSRCLKAHGLPTSMDDPLIRKTAKAARLNVETLLDI
MRVDKKNSGNVKKVVILSRIGKTLEERATGVKDEVIARVLAPAVRVYPGPPTKQTFELAT
PGSKSISNRALVLAALGNGTCKLGNLLHSDDTQVMMAALNEMKGAEFAWEDNGELLVVKG
NGGKLSPPKDSKELYLGNAGTAARFLTTVCALVQPEGDLQHTVITGNARMKQRPIGPLVD
ALTANGTGVEYVENQGALPLKIAATEAGFRGGKIQLAASVSSQYVSSILLCAPYAREEVI
LELTGGVVISQPYIDMTIAMMASFGIRVERLKGDDGKLLDTYRIPRGTYVNPPTYNIESD
ASSATYPLAMAAITGTTCTIHNIGSASLQGDARFAKDVLEPMGCKVVQTETETTVTGPPV
GQLRALGFVDMEPMTDAFLTASALAAVASLPPLEGRLQDADQPLNSTRIGGIANQRVKEC
NRIDAMRTQLAKFGVQTNELEDGIEVFGIKPEQLRAGASVHCFDDHRVAMAFSVLAACPG
AHGAVLEEKRCVEKTWPSWWDDLSRKIGIEVIGVELDDSPVASTSAAIPRHSTDASIFVL
GMRGAGKTHISRIGASALGWPVLDADEMFITEYGQSAKDFVNLRGGDWSEFRVAETHILK
KIIREYSKGHVISLGGGVVETEENRSLLRAYGHGGGPIVHIIRDIDDIVGYLNSEPSRPS
LGEDLHVIYARRRPWFHELSNFEFTNLISGKPKAHKQGVKPTANGFIVSHTSTKGAEDEV
ARFFRFMTGQNTNHVTLAGARPTYFLCLTLPSYTAPHPALEQFDELIAGVDALELRVDLL
SADGKTPTKPQIPDYDYVAVQLAALRQRSTLPIIFTVRTVSQGGMCPDEAQDEIFKLMEL
GVKSGCEYVDLEVRWPEKRMREFVGIKQSTKIIASWHDWSGNLHWESEEAAQRYKAANEV
GDIIKIVAKANSLLDNLTMLRFREMFKDGKPLMTMNIGDDGRLSRILNPVFGPVSHPAIT
ASAPGQLSFAQIQTGLHLIGHLPARKFYLVGSPIQHSKSPLIHNTAFKVLGLPHHYGLIE
SEQVNDDIKRAIRAPDFGGASVTIPLKLDIMPLLDEVSEHARLIGAVNTIIPVEEHGARR
LIGDNTDWLGLLELIEKNLSSDNERTDDSTSLVLGAGGTCRAAVYALHKAGFKTIYLFNR
TRPNADKIVESFPKEYNIVPLTSLDSFPGEHPLAVISTIPAQGTATKYAPNPDAGVPVPD
SVLAREHGGVLIDVAYKPKITPLIDLADRTPGWTGVPGIQMLLEQGFWQSAMWTGRRPPK
EIIRRAVLTEYDRDN