Protein Info for mRNA_6118 in Rhodosporidium toruloides IFO0880

Name: 14486
Annotation: K03164 TOP2 DNA topoisomerase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1577 PF02518: HATPase_c" amino acids 174 to 318 (145 residues), 51.1 bits, see alignment E=4.7e-17 PF00204: DNA_gyraseB" amino acids 424 to 558 (135 residues), 88.7 bits, see alignment E=8.3e-29 PF01751: Toprim" amino acids 589 to 687 (99 residues), 23.8 bits, see alignment 1.1e-08 PF16898: TOPRIM_C" amino acids 703 to 829 (127 residues), 177.8 bits, see alignment E=2.7e-56 PF00521: DNA_topoisoIV" amino acids 831 to 1285 (455 residues), 442 bits, see alignment E=6.2e-136

Best Hits

Predicted SEED Role

"DNA topoisomerase II (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent (EC 5.99.1.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1577 amino acids)

>mRNA_6118 K03164 TOP2 DNA topoisomerase II (Rhodosporidium toruloides IFO0880)
MPRYASSDEEDDFHMSDSGSDFEGAGAGKKGKKAALKPAPKKTPAATKKAPAAKKTTTTV
AGGGKGKGKKVAAPLEEIDTNGGRGAASDEEEMEEDAMEEDDDDDFDTGKKAPAKGKGKA
KSASETYVKLTQLEHVLKRPDTYIGSIEPVTQPMWVWDRESEKMVFRQTTFTPGFYKIFD
EILVNAADNKVRDPTMSEIRVTIDEEKRTITVKNDGKGIPIEKHKKEGIYIPELIFGNLL
TSSNYDDDEAKTTGGRNGYGAKLANIYSTEFIVETADGESKYRQVFSDNMSRKGAPKITS
VKKDGEQYTLITFTPDLERFNMHDGFDRDTLALLHKRVWDMAGIVPKVKVYLNGKVLRCK
DFKAYVEKFLAGTNEITGAAAAKKKGKKGEAPVTAAAALFGDEEEDADMRPAADADAAKQ
TVVFEKVNDRWDVAFAVSDGQFQQVSYCNAIATTKGGSHVTYVADQIVQGLVDLVKKKHK
GVQVKPFQIKNHIALFVNCKIDNPSFDSQTKENMTLGVKKFGSKCQLSDEFLKKVAKSGV
IDNIVNWAKFKQDQALKKTDGSKRSRITGLVKLEDANNAGTRNGNKCTLILTEGDSAKAL
AVSGLAVVGRDNYGVFPLRGKLLNVREATGKALLENAEIQAIKQIMGLQQGKTYTSTDSL
RYGSLMIMADQDHDGSHIKGLIINFLDYWFPSLLKLPNFLVEFITPIVKVSKGKKEISFF
TIPEYEAWKAETDDGRGWKIKYFKGLGTSDTNDAKKYFSDMNRHRLPFKELTPEDRALID
MAFNKKKADDRKEWLRGFVPGTYMDHDVDEVPIKDFINKELILFSMADNIRSIPSVVDGF
KPGQRKVLFGCFKRKLKNEIKVGQLSGYISEHSAYHHGDASLQGTIVGLAQNFVGSNNIN
VLDPNGQFGTRLQGGKDAASARYIFTNVAQITRTLFHPSDDAILDYLNDDGQSIEPSWYI
PILPMVLVNGSDGIGTGWSSFIPNFNPSDIVANLRRKLDGEEMEPIHPWYRGFRGTIEKD
AKEGYKCTGTIQRIDDSTVEITELPIRTWTQNYKEMLESWVAGSDKQAAWCKDYKEYHTD
TTVHFVVTLTTAGKEAIEKDGLEKVFKVTSKLNTSNMVCFDPTGKIKKYATAEDIVSDFY
DVRLEFYHKRKKHLVSELELVHDRLSNQARFIKMIIDKQLVLSNRKRADIVAELRKKGFR
PFPKVQKMHVAGDVDANGDGDEEVEDPEAVEAGADSDYDYLLNMQLSSLTREKVERLMAE
RDQRSAELDALLKRSVQDLWRADLDAFMEKWDAMLEEDRANKARSLKKAKSAGKGTAKKR
AAYASDDDDDFEVKPKKKAPAPKKAKPAVPLPETDDDEDVVMKAAAKPKAAAAAKPKAPA
KRKAASIDLDLSDEDGGAAEKDETPPKKKKATAAAGTAGKKAAASKKKAAASDIEDEDDE
LDDLYALPAKKPAAHSPKGKKATASKAKAKSVEVDDSDDEPVVAKKKAPAKAKAPPAKKA
AAPKKKKSAASDDDDDLDLDLSMSDAPAPKARAARSTRAPAKSYQLDLGSDFEGDESFQV
EDDEDEEDAFELGDESE