Protein Info for mRNA_6229 in Rhodosporidium toruloides IFO0880

Name: 14597
Annotation: K01895 ACSS, acs acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 TIGR02188: acetate--CoA ligase" amino acids 28 to 655 (628 residues), 1002.6 bits, see alignment E=3.1e-306 PF16177: ACAS_N" amino acids 33 to 89 (57 residues), 73.1 bits, see alignment 2e-24 PF00501: AMP-binding" amino acids 91 to 530 (440 residues), 318.9 bits, see alignment E=6.5e-99 PF13193: AMP-binding_C" amino acids 539 to 621 (83 residues), 91.1 bits, see alignment E=1.1e-29

Best Hits

Swiss-Prot: 74% identical to ACSA_COPC7: Acetyl-coenzyme A synthetase (ACS-1) from Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 70% identity to scm:SCHCODRAFT_77294)

MetaCyc: 62% identical to acetyl CoA synthetase 2 (Saccharomyces cerevisiae)
Acetate--CoA ligase. [EC: 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (660 amino acids)

>mRNA_6229 K01895 ACSS, acs acetyl-CoA synthetase (Rhodosporidium toruloides IFO0880)
MADVDYKAVKTHPISKRNDGSTGHKPHSNEEEYKKLYERSVKDPLGFWDEMAKEHLYWHR
PYTTVQAGSFTAGDVQWFPEGGLNVSYNCVDRWAYKHPNKTAIIWEADEPGEHVELTYEQ
LFQEVCRTANILKSYGVKKGDTVAIYLPMVPEAAIAFLACARLGAIHSVIFAGFSAESLR
DRIVDAKSRVVITTDEGKRGGKTIATKSIVDAALTECPLVEHCLVLKRTGGNIKWTEGRD
HWWHEEKDKVQPYCPVEIVSSEDPLFILYTSGSTGKPKGVVHSTAGYLLGAFMTLKYVFD
VHPEDRYACMADVGWITGHTYIVYGPLANGVTTTIFESTPVYPTPSRFWEVVAKHKLTQF
YTAPTAIRLLRRLGEEHTKGHDLSTLRTIGSVGEPINPEAWEWYWEHVGKKECAVVDTYW
QTETGSIIITPLPGATKTKPGAATLPFFGIDPVLLDPTTGKEISGNDVEGVLCVRKPWPS
IARTVFGDHKRFLDTYMNVYPGYYFTGDGAGRDHDGYYWIRGRVDDVINVSGHRLSTAEI
ESALIQHNGVAETAVVGIPDELTGQAVVAYVTLKPDFSFDPSDEAALSKELVLQVRKTIG
PFAAPRKLVLINDLPKTRSGKILRRLLRKISSGEGDQLGDLSTLADPSVIDEIKAKLSSE