Protein Info for mRNA_6437 in Rhodosporidium toruloides IFO0880

Name: 14805
Annotation: K07511 ECHS1 enoyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 47 to 290 (244 residues), 280.1 bits, E=1.5e-87
• PF16113: ECH_2
  • amino acids 50 to 217 (168 residues), 116.2 bits, E=2.3e-37

Best Hits

Swiss-Prot: 58% identical to ECHM_DICDI: Probable enoyl-CoA hydratase, mitochondrial (echs1) from Dictyostelium discoideum

KEGG orthology group: None (inferred from 71% identity to uma:UM04973.1)

MetaCyc: 57% identical to short-chain enoyl-CoA hydratase monomer (Homo sapiens)
Enoyl-CoA hydratase. [EC: 4.2.1.17]; 4.2.1.17 [EC: 4.2.1.17]; 4.2.1.17 [EC: 4.2.1.17]; RXN-21770 [EC: 4.2.1.17]

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Butanol Biosynthesis or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 4.2.1.17)

MetaCyc Pathways

• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• oleate β-oxidation (26/35 steps found)
• fatty acid salvage (5/6 steps found)
• valproate β-oxidation (7/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (4/5 steps found)
• L-valine degradation I (6/8 steps found)
• L-isoleucine degradation I (4/6 steps found)
• methyl ketone biosynthesis (engineered) (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• glycerol degradation to butanol (11/16 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• fatty acid β-oxidation I (generic) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (4/9 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (1/5 steps found)
• superpathway of phenylethylamine degradation (5/11 steps found)
• benzoyl-CoA degradation I (aerobic) (2/7 steps found)
• phenylacetate degradation I (aerobic) (3/9 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (4/11 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
• L-tryptophan degradation III (eukaryotic) (6/15 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (4/13 steps found)
• L-glutamate degradation VII (to butanoate) (3/12 steps found)
• gallate degradation III (anaerobic) (2/11 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (4/14 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (5/18 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• platensimycin biosynthesis (6/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (3/22 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.2.1.17

Protein Sequence (291 amino acids)

>mRNA_6437 K07511 ECHS1 enoyl-CoA hydratase (Rhodosporidium toruloides IFO0880)
MLPALRATSMRTALPRFPRQSVVRWQSSAAAGPGEYTSILVSQPAPGVSQITLNRPKALN
ALNSTLFHELNAALRTLSSDDSTGCVVLTGSDKAFAAGADIKEMKDKQFAEVYKGDFLAH
WTELSGFRKPIVAAVSGYALGGGCELAMMCDIILAAPNAVFGQPEINLGVIPGAGGTQRL
THALGKSRAMEIVLTGKNFSAAEAAEWGLVSRVVQEGAVVDEAVKVAGKIAGKGMLAVQA
AKEGVNAAYEMSLTNGLHFERRLFHQLFATNDQKIGMNAFANKEKAKWTNS