Protein Info for mRNA_6769 in Rhodosporidium toruloides IFO0880

Name: 15137
Annotation: K01953 asnB, ASNS asparagine synthase (glutamine-hydrolysing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 410 (409 residues), 339.1 bits, E=2.9e-105
• PF13522: GATase_6
  • amino acids 34 to 166 (133 residues), 105.1 bits, E=5.2e-34
• PF13537: GATase_7
  • amino acids 49 to 171 (123 residues), 126.7 bits, E=7.5e-41
• PF00733: Asn_synthase
  • amino acids 220 to 404 (185 residues), 193.7 bits, E=1.2e-60
  • amino acids 403 to 569 (167 residues), 130.1 bits, E=2.5e-41

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 62% identity to ncr:NCU04303)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• superpathway of L-citrulline metabolism (10/12 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-asparagine biosynthesis (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-asparagine biosynthesis II (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (3/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (605 amino acids)

>mRNA_6769 K01953 asnB, ASNS asparagine synthase (glutamine-hydrolysing) (Rhodosporidium toruloides IFO0880)
MCGIFAAHAYDPKDLPSFRARAVALSKRLRHRGPDWSGCKMTQDAILVHERLAIVGVDTG
AQPITSDDDKLVLAVNGEIYNHRALRKQLKNPGTFKTHSDCEVILHLYKEHDKDLCAMLD
GMFSFVLVDTSVSPSRLIAARDPIGITTLYQGWNSQRPGTVYFASELKALHDECDKVISF
PPGHVYDSATQETTRYFQPSWWNGDVDPKAVPTKPADLDELRTKLEKAVKKRLMSEVPYG
VLLSGGLDSSLIAAIAARETEKVAAAQQAHQHQGKQGSAGDASGLVGVDDEAAADEVGTL
TSWPRLHSFSIGLEGSPDLLAARVAAEYLGTVHHEYTFTVQEGLDAVEEVIYHLETYDVT
TVRASTPMYLLSRKIKAMGVKMVLSGEGSDEIFGGYLYFYAAPTAADFHQECIRRVKNLH
TADCLRANKSTMAWGLEARVPFLDKEFLETALNIDPQQKMFSKGSLQERDADGRPIMEKY
IIRKAFDVGPKGERPYLPDEILWRQKEQFSDGVGYSWIDGLKDHAAQVVTDEQMASAAQR
WSLDTPETKEAYFIRQIFESHFPSEAAAKTAVRWIPRADWGCASDPSGRAVQIHESAYDG
KATQA