Protein Info for mRNA_6932 in Rhodosporidium toruloides IFO0880

Name: 15300
Annotation: K00764 purF, PPAT amidophosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR01134: amidophosphoribosyltransferase" amino acids 2 to 471 (470 residues), 455.2 bits, see alignment E=1.6e-140 PF13522: GATase_6" amino acids 63 to 164 (102 residues), 48.3 bits, see alignment E=2.5e-16 PF13230: GATase_4" amino acids 72 to 186 (115 residues), 23.8 bits, see alignment E=4e-09 PF13537: GATase_7" amino acids 84 to 200 (117 residues), 35.5 bits, see alignment E=1.8e-12 PF00156: Pribosyltran" amino acids 276 to 400 (125 residues), 37.1 bits, see alignment E=4.7e-13

Best Hits

Swiss-Prot: 55% identical to PUR1_YEAST: Amidophosphoribosyltransferase (ADE4) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 68% identity to scm:SCHCODRAFT_81451)

MetaCyc: 55% identical to phosphoribosylpyrophosphate amidotransferase (Saccharomyces cerevisiae)
Amidophosphoribosyltransferase. [EC: 2.4.2.14]

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (566 amino acids)

>mRNA_6932 K00764 purF, PPAT amidophosphoribosyltransferase (Rhodosporidium toruloides IFO0880)
MCGILAVLLASQNNVASEIAEGLQLLQHRGQDACGVVTCGPKGRFYQCKGNGMVRDLLDA
KNLSQLIGGMGVGHARYPTAGSSAQSEAQPFYVNSPYGLVFAHNGNLINTDHLRYYLDHY
AHRHINTSSDSELLLNLLADALQKTGKFRIDEDDIFRAIREVMQMTSGGYACTAMIAGYG
IFGFRDPNGIRPMGWAKRKSETVPGGFDYMMASESVVCDALGYSDWTDIKPGEAIIVTQD
GVKTMQCTPARTFAPDVFEYVYFARPDSVVDGISVYRARMAMGDALAATAKAQLDKAGIK
VDVVIPVPDTSRVAALQLAQNLNIPYREGFVKNRYVGRTFIMPGQELRRKNVRRKLNAMA
LEFAGKNVMIVDDSIVRGTTSKEIIVMAREAGAKKVIMASCAPPIRYPCVYGIDMPSRHE
LVAHDRTEDEIAEYIGADHIIFQSLDDLVSSCQRLNPSIERFECSVFNGEYVTGGVDEAY
LSHIEGLRNDNAKRKAGQQGAGPALVNINGANASNVVLASQPLGDVTGGGEVAGGGDISQ
VVGLSNENAGEAKESPSIVVGLDNQR