Protein Info for mRNA_6998 in Rhodosporidium toruloides IFO0880
Name: 15366
Annotation: K00288 MTHFD methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to C1TC_SCHPO: C-1-tetrahydrofolate synthase, cytoplasmic (SPBC839.16) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)
KEGG orthology group: K00288, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase [EC: 1.5.1.5 3.5.4.9 6.3.4.3] (inferred from 68% identity to scm:SCHCODRAFT_81176)Predicted SEED Role
"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.5, EC 3.5.4.9, EC 6.3.4.3)
MetaCyc Pathways
- folate polyglutamylation (5/5 steps found)
- formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
- tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
- folate transformations III (E. coli) (7/9 steps found)
- formaldehyde oxidation VII (THF pathway) (3/4 steps found)
- folate transformations II (plants) (8/11 steps found)
- folate transformations I (9/13 steps found)
- reductive glycine pathway (6/9 steps found)
- superpathway of tetrahydrofolate biosynthesis and salvage (8/12 steps found)
- purine nucleobases degradation II (anaerobic) (14/24 steps found)
- L-histidine degradation III (1/6 steps found)
- reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
- purine nucleobases degradation I (anaerobic) (5/15 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (26/57 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.5.1.5, 3.5.4.9
Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9 or 6.3.4.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1010 amino acids)
>mRNA_6998 K00288 MTHFD methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase (Rhodosporidium toruloides IFO0880) MLARSHQVALRAAQSSTRILARTAVASLSQRAFTAGTASLSPSPRRLTPLRSSPSPSRSL ATMAEQNQGAKLIDGNAIAASLRQGIAARIAETRARFPRFEPTLVIVQQGARPDSSTYVK MKLKAAAEAGIKCTLVQLGGPEDGVGENEVMAEVERLNADSDVHGIIVQLPLSDEIGRDG ERRITEAVSPEKDVDGFHAYNIGLLSSRASEPLFAPCTPAGVMLLLESTGIEISGKNAVV LGRSDIVGSPVCALLRRKDATVTQCHSRTKNLADIVRQADIVVAAIGQARFVQGDWIKPG AVVIDVGTNYIADSTRKSGSRLVGDVDFDSARQVASYITPVPGGVGPMTVAMLMENTVLS AQRLLEKSQQRLVKPLKLVCKEKVPSDIEIAKSQVPKPIDVLAAEIGIPAKDLELYGRNK AKVKLEVLDSLRHRKDGKYIVVAGITPTPLGEGKSTTTIGLAQALGAHLGKAAYACVRQP SQGPTFGIKGGAAGGGYSQVIPMDEFNLHLTGDIHATTAANNLLAAAISARYFHESTQSD KALFSRLCPPKKGVRSFSPVMIKRLEKLGIDKRNPDDLTEEERSAFVRLDIDPEKISWHR VLDTNDRYLRKVMTGLGPAEQGKTLETGFDIAVASECMAVLALATSLGDMRDRLGRMVVA ESKSGVPITCDDIGATNSLAILMKDAIKPNIMQTLEGTPVFVHAGPFANIAHGNSSILAD KIALKLAGIEEGEEEDKNGYVITEAGFGADIGMEKFVNIKCRASGLIPNAVVLVATIRAL KMHGGGPEVTPGKPLPEVYLNEDLDILKAGCANLARHIENAKKVGVKVIVAVNRFTSDTP AEIKLVQEQALAAGADAAVPCNHWAEGGKGAIELGEAVIQACKEPNPFKFLYDVNLPIKD KIEIIAKEFYGAAAVEYSELAESQIKSFEETGYGKLPICMAKTHLSFSADPKLKGAPSGF TIPIREARLSAGSGFIYPLVGEMSTMPGLATRPCFFDHQLLDDGEIIGLS