Protein Info for mRNA_7053 in Rhodosporidium toruloides IFO0880

Name: 15421
Annotation: K01192 E3.2.1.25, MANBA, manB beta-mannosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 899 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00703: Glyco_hydro_2" amino acids 293 to 335 (43 residues), 26 bits, see alignment 1.3e-09 PF17786: Mannosidase_ig" amino acids 725 to 820 (96 residues), 38.3 bits, see alignment E=1.8e-13

Best Hits

Predicted SEED Role

"Beta-mannosidase (EC 3.2.1.25)" in subsystem Mannose Metabolism (EC 3.2.1.25)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (899 amino acids)

>mRNA_7053 K01192 E3.2.1.25, MANBA, manB beta-mannosidase (Rhodosporidium toruloides IFO0880)
MRVQSLSSPLLWSWRQAPSTYDASLSLSSSSSAPQQLSLSQHEGGFRPVAHFPSVIHEEL
LAVGEIPDPFLRRNEEAVQWVGEADWIYRCEFEVERLPKKRSKNGDGEEERADLVFEGLD
TFATVYLNGDKILEANNMFREWRVPLQRSQLRHGRNSLYLVFHSAFRRGRELQMQILGRN
KSWPAWNGDPSRLFVRKAQYHYGWDWGPTLMTAGPWRPVRLEFYRSRIVDFYPRASVSAT
LEPALSLSWKVADSNDNLACVTRLLSPCGDVLRKADHSANLRSDEQHEWRFADGEVDLWW
PAGMGEQPLYEVGVDLVDMTGGEVLDSVTRHVGFRRLRVVRDPLHHPPQPGHTFLCEVNN
VPFFVGGSNWIPIDSILTRATKKRYRKWLELAKAGNQKMVRVWGGGIYEDEAFYKLCDEL
GLLVWQDFMFACGAYPAQLNDFRANVEAEVEAVVKRLRAHPSLAIFAGNNEDYQIAEAEK
LEYDPKDEKGPTGNWLRTNFPARELYERVFPRIVKENSDTFYWPGSPWGGKSTRDQTEGD
VHVWDVWHGNQLPYQEYGQLSGRFVSEFGMQGAPDIRTIDAFLAGDTSERFPQSRTMDAH
NKAVGHERRLAAYITENIRAGTSLEDYVYATQFIQAEAVYSAFSSWRRKFYGGVGGAECA
GALVWQLNDVWPCTSWSIVDYYLRPKLAYFTMKRALASLAIGGRRFTEKTYPDPTSAADF
LEKTHVEFWVASSELEPREVEIKSVGYELSTGKRRVSGRMLDERWTQVVGPNEATRLKRC
TIPNDWNNAESAVVVFGWLFDPETGETLSRVSLWPEPFKYLNFPTEADTDISINLFRHGQ
DAGEIRITAQRPVKGLVLTLDPEAQLDDNFLDLAPGDEQVVKVKGLAKGTKVSWRHLGD