Protein Info for mRNA_7120 in Rhodosporidium toruloides IFO0880

Name: 15488
Annotation: K01649 leuA 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 614 TIGR00970: 2-isopropylmalate synthase" amino acids 32 to 595 (564 residues), 791.9 bits, see alignment E=1.6e-242 PF00682: HMGL-like" amino acids 58 to 345 (288 residues), 252.1 bits, see alignment E=7.4e-79 PF08502: LeuA_dimer" amino acids 457 to 592 (136 residues), 100.4 bits, see alignment E=7.6e-33

Best Hits

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 56% identity to lbc:LACBIDRAFT_244298)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (614 amino acids)

>mRNA_7120 K01649 leuA 2-isopropylmalate synthase (Rhodosporidium toruloides IFO0880)
MFARSALARSANIVTRSMSSTTVGRSQWLKDPSVKYVPFQSVNLPDRTWPDKRITKAPRW
ASSDLRDGNQALVNPMTIEQKQRVFNLLLKCGFKEIEAGFPSASETEFAWIRGLIEKNHC
PDDVWLQVLSPAREELIRRTFEALRDAKNVIFHMYNAAAPVFREQVFGNTQQQTIDLAVK
HVKLVRQLVDEAVARGDRTNWQFEYSPEAFSQTEPEFAVELCNAVQEAWFAGKDKSKEAP
IIFNLPATVEVATPNNYADQIEYFCRNIKERQHVVVSLHTHNDRGTGVAASELGLMAGAD
RVEGCLFGNGERTGNVDLVTLAMNMYTQGVSPELDFSNLEECIEVITSCTDIPVHPRAPW
AGELVFTAFSGSHQDAIKKGFHYRKKDESRPWSIPYLPVDPADIGMTYEAVIRVNSQSGK
GGVAYLVQRALQLDLPKKMQPAFYAVVQSISERTAKEITAEDIEKAFRAAYYVGKDYNGR
FNLNDYSFATGQDGKKTFKGSITDNGDKKSIEGDGNGPVSALLNALNSTWDGGKHNLEVK
EYSEHAIGAGSDVKAASYVQLVDKTSGRSVWGVGVSTDVTEASLKAVLSAASNAAVASDR
RYTEIEDIVIGGRV