Protein Info for mRNA_7263 in Rhodosporidium toruloides IFO0880
Name: 15631
Annotation: K00615 E2.2.1.1, tktA, tktB transketolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 64% identical to TKT1_YEAST: Transketolase 1 (TKL1) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 68% identity to cnb:CNBD5360)MetaCyc: 47% identical to transketolase 2 (Escherichia coli K-12 substr. MG1655)
Transketolase. [EC: 2.2.1.1]; 2.2.1.1 [EC: 2.2.1.1]
Predicted SEED Role
"Transketolase (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)
MetaCyc Pathways
- Bifidobacterium shunt (14/15 steps found)
- formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
- pentose phosphate pathway (8/8 steps found)
- pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
- pentose phosphate pathway (partial) (3/3 steps found)
- Rubisco shunt (8/10 steps found)
- superpathway of glucose and xylose degradation (13/17 steps found)
- pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
- formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
- Calvin-Benson-Bassham cycle (9/13 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
- ethene biosynthesis V (engineered) (16/25 steps found)
- oxygenic photosynthesis (10/17 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (16/26 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of ansamycins
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Pentose phosphate pathway
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.2.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (687 amino acids)
>mRNA_7263 K00615 E2.2.1.1, tktA, tktB transketolase (Rhodosporidium toruloides IFO0880) MPFQPNDNDQKAINTIRTLALDTVNKANSGHPGAPMGMAPVAHVLFSRFFQCNPQNPHWV NRDRFVLSNGHACALQYVLLHLLGYKVSIDDLKQFRQLESITPGHPELGVTEGIEVTTGP LGQGFANAIGLAIAQAHLGAVFNKDGYSLFDNYTYMFTGDGCLMEGVSAEAASLAGHLKL GNLIAIYDDNQISIDGSTAIAFTEDVGKRFEAYDWQVLHVENGNDDLKAISDAIEQAKAE KNKPTLIRLKTTIGYGSRDQGTHGVHGNPLKADDTVEIKKKFGFDPEQFFVVPDETKKIY SDIAQRGAEAEKKWQAQFNEYEQKFPEEAKDIKRRIDGKLPDGWEKALPEFKPSDDAVAS RKLSEMILAKLSTVVPELVSGSADLTPSNLTRWKNAVDFQPKENGLGDYTGRYIRYGVRE HGMGAIANGIAAYGGNLVIPAIGTFLNFVSYAAGAVRLAALSHLRVIWVATHDSIGLGED GPTHQPIETVAHFRALPNCNVWRPADGNETSASYYVAMTSTHTPSILCLTRQNLPQLAGS SIEKASKGGYVLHEVENADVTLVATGSEVAIAVEAAELLEKQGLKARVSSLPCWEVFRAQ SQEYKLSVLPDGAPIVSVEAYTTFGWAEFSHAAVGIDRFGASGPARDVYAKFGLTGKDIA RRAQKTIQYYKDLGHPVYSPLSTAIHA