Protein Info for mRNA_7648 in Rhodosporidium toruloides IFO0880

Name: 16016
Annotation: K01705 LYS4 homoaconitate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00139: homoaconitase
  • amino acids 25 to 480 (456 residues), 693.1 bits, E=2.2e-212
• PF00330: Aconitase
  • amino acids 30 to 471 (442 residues), 368.5 bits, E=6e-114
• PF00694: Aconitase_C
  • amino acids 584 to 644 (61 residues), 64.6 bits, 1.2e-21

Best Hits

KEGG orthology group: K01705, homoaconitate hydratase [EC: 4.2.1.36] (inferred from 67% identity to lbc:LACBIDRAFT_184939)

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-lysine biosynthesis IV (5/9 steps found)
• L-lysine biosynthesis V (4/10 steps found)
• coenzyme B biosynthesis (4/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Lysine biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.36

Protein Sequence (727 amino acids)

>mRNA_7648 K01705 LYS4 homoaconitate hydratase (Rhodosporidium toruloides IFO0880)
MYALRARSAGTLPLRRYLATHAKLPQTYIEKVVQRHSVGLPEGKVVRAGDYVMIKPQHVM
THDNTGPVISKFKSIGATKIANPRQTVFTLDHDVQNRSPANLSKYAKIEAFAGDHEVDFY
PAGRGIGHQIMIEEGYAFPGVMTVASDSHSNMYGGVGCVGTAIVRTDAAALWATERTWWQ
VPHIVRVNLEGKLPPGVTGKDIIIALCGAFNKDEVLNHAIEFHGSGIPQLSVDERLAIAN
MTTEWGALVGVFPTDDVLKKWYESQLKKLELRRFEFGGSASGSLSSSTSQHPRLNPQRLE
QVFANPVLPDEGAYYAKTLTLDLTSLSPSVAGPNSVKVATPLPVLAKEDIKINKAYLVSC
TNSRASDIKAAADVMRGKKVAPGVEFYIAAASSVVQKEAEKAGDWAALVSAGAKVLPAGC
GPCIGLGTGLLEDGEVGISATNRNYKGRMGSPKALAYLASPEVVAASAVAGKITGPQVAG
ATPTEDAPRISIEEHASSASASTSSAVAEPLLPNFPPLFSGPLLFAPQDNLNTDGIYPGK
YTYQDDITREKQADVVMENYDPAFASIMKSLDSPARSTNPAGPQRNGDGVLLASGYNFGT
GSSREQAATALLSSGIPLVLCGSFGDIFKRNSINNALICVECPELIEDLTRDFALDGKRG
AGGKDGKLTVQTPYWVEVGSVDGKVTLRKEKGGEVVKEYKVGAVGGSVQEMWLAGGLEGW
VKERISA