Protein Info for mRNA_8247 in Rhodosporidium toruloides IFO0880

Name: 16615
Annotation: K01426 E3.5.1.4, amiE amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 53% identity to pno:SNOG_02612)

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

superpathway of L-citrulline metabolism (10/12 steps found)
L-citrulline biosynthesis (7/8 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
L-glutamate and L-glutamine biosynthesis (3/6 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>mRNA_8247 K01426 E3.5.1.4, amiE amidase (Rhodosporidium toruloides IFO0880)
MPVPLDLARLDASEALALIRKDEVTVTQYVQALISRIDERDPVVRAWAYFDPAYVLEQAR
ALDALPREQRGPLHGLPVGVKDIMLTKDMPTQYRSPIYSSKVPIGMDSAPVMTLRAQGAL
MLGKTTTTEFATCHRGCETVNPHDTSRTPGGSSSGSGAAVADFQVPVALGTQTGGSVIRP
ASFNGIYGLKPTWNAISREGVKIYSLTCDTVGFFSRSITDLQLLSSAFQIRDDAAPPASP
LSLKGAKFGFVKTCVWPRATEGTVKAFERAKELLEAEGAVVEEVELPKEFDEIPRLYQYV
LSSEGRSAFLGDYLLNPSKLDLLILSHSRNDLHVTRKKHLEGYDKIAALRPIIDEIARKY
DALVTPSTTDEAPVLVEPERHTGEAVFNLMWTILHVAVLNLPGFAGPAGCPLGLSLVAPR
FEDERLLHVGEAVGRVFAEKGGWASAL