Protein Info for mRNA_8298 in Rhodosporidium toruloides IFO0880

Name: 16666
Annotation: K02858 ribB, RIB3 3,4-dihydroxy 2-butanone 4-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 56 to 254 (199 residues), 246.3 bits, E=1e-77
• PF00926: DHBP_synthase
  • amino acids 59 to 253 (195 residues), 289.1 bits, E=6.8e-91

Best Hits

Swiss-Prot: 49% identical to RIBB_KLEP7: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K02858, 3,4-dihydroxy 2-butanone 4-phosphate synthase [EC: 4.1.99.12] (inferred from 63% identity to scm:SCHCODRAFT_15318)

MetaCyc: 46% identical to 3,4-dihydroxy-2-butanone-4-phosphate synthase (Escherichia coli K-12 substr. MG1655)
3,4-dihydroxy-2-butanone-4-phosphate synthase. [EC: 4.1.99.12]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (6/9 steps found)
• flavin biosynthesis III (fungi) (6/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (2/5 steps found)
• flavin biosynthesis II (archaea) (4/10 steps found)
• toxoflavin biosynthesis (1/7 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Protein Sequence (259 amino acids)

>mRNA_8298 K02858 ribB, RIB3 3,4-dihydroxy 2-butanone 4-phosphate synthase (Rhodosporidium toruloides IFO0880)
MAAVVAGSAAALAGAPAHTAARLASSNEPSSSSSASAPEQVTISDTTPSGRTVELDSVED
ALEAFRKGDFLVVVDDMDRENEGDLIIAANRITQQQMAWLIRHSSGYVCICLPQRRLAEL
DIPMMVEDNKEKHRTAYTITCDYKHGTTTGISAHDRALTARKLADSSSKAEDFTRPGHLV
PLRAVDGGVLVRRGHTEASVDLCRLAGLPEAGVICELVKPDDPNGGMARRDDCFAFARQW
GLKMISIEQLEQYRREKGL