Protein Info for mRNA_178 in Rhodosporidium toruloides IFO0880

Name: 8546
Annotation: K00974 cca tRNA nucleotidyltransferase (CCA-adding enzyme)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 659 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF01743: PolyA_pol" amino acids 91 to 228 (138 residues), 76.2 bits, see alignment E=3.1e-25 PF12627: PolyA_pol_RNAbd" amino acids 284 to 329 (46 residues), 26 bits, see alignment 6.4e-10

Best Hits

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.21, 2.7.7.25

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (659 amino acids)

>mRNA_178 K00974 cca tRNA nucleotidyltransferase (CCA-adding enzyme) (Rhodosporidium toruloides IFO0880)
MLAFLARLPSHRLILAKTPLRRLSFTRAAMSTVSPDLPTITLTPNEAKLVQLLTDCADWV
DQHPEEVDALRLKDDQGRWIGRERGTEAVELRVAGGWVRDKLLGRQSDDIDVSTSPDPIT
GLKFAMLFEKYLETLGQRDLMGRLTKIEAKPEQSKHLETATARVCDLSVDWVQQRGQEVY
TEGSRIPTVAFGTPLEDAERRDLTINSLFYNLRTRLIEDQTGKGLSDLGFVPGQPKKIRT
PLEPVKTFRDDPLRVVRAVRFAARFGREYELDTEMAEAIKREEIKEALRNPLKISRERVG
AELDKMLLGKDPHYAFELIESLELHPLIFLYEHDKCFPMPSTPSSPTASPSLSSSDPSAS
PAPEPHTSISVVAASLLTQLLNPSSSSLPALHPLLQLHSVPLPDTTNAHSAPPTYPPVSP
SPSTPFLTLVYPPTVKRLYLSCGLLPLYYLGTREKSKLVWVGEKVVREGVKGPSVDVGFV
KRVREASEVLRGLVREVSGRSEGTEERMVDEAEERATIGMHLRNPSIHDGQHQRWNVSLL
WSLVVDLVSARSDPAEQARLVEMYNRFVEKVVSYELDRRAFEHPLLDGKTVNTLLPFTRS
SPAMPRILDLIMRFQLAHPSATKEVAERLLCEEERVEGLRRLVEESRPQPKGKKRKEPK