Protein Info for mRNA_201 in Rhodosporidium toruloides IFO0880

Name: 8569
Annotation: K00294 E1.2.1.88 1-pyrroline-5-carboxylate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 TIGR01236: 1-pyrroline-5-carboxylate dehydrogenase" amino acids 15 to 552 (538 residues), 771.8 bits, see alignment E=1.8e-236 PF00171: Aldedh" amino acids 62 to 536 (475 residues), 335.4 bits, see alignment E=2.5e-104

Best Hits

Swiss-Prot: 60% identical to PUT2_AGABI: Delta-1-pyrroline-5-carboxylate dehydrogenase (pruA) from Agaricus bisporus

KEGG orthology group: K00294, 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12] (inferred from 62% identity to cci:CC1G_10646)

Predicted SEED Role

"Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization (EC 1.5.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>mRNA_201 K00294 E1.2.1.88 1-pyrroline-5-carboxylate dehydrogenase (Rhodosporidium toruloides IFO0880)
MSVSTNAIFKLPTVKNEPNFNYAPGSPERAALKKALAELEAAAPFEVPAFVGGKEVKVSG
ERTEQPMPHNHKKALCTFGTSTPELASQAIEAALAAQPEWEAMPFNDRAAIFLRAADLIS
GKYRAKICAATMLGQGKNVWQAEIDAAAELADFFRFGAAQVEQLYAMQPTENSPGVWNRT
EYRPLEGFVFAVTPFNFTAIGGNLVGAPALTGNVLVWKPSPMATYSSWLVFQILLEAGLP
KNVIQFLPCPNGDATISLVDRVLSHRMFAGLHFTGSTHVFRHLWQKIGNNINNYLSYPRI
VGETGGKNFQLVHPSANVRAAVTGAIRGAFEYQGQKCSALSRLYVPKGLWEGEGKFKEIL
LSEVAKITLGPVTEFEHFMGPVISQASFDKCLSYVEKAKQAGGEVLAGGKGDASSGYYVE
PTIILTKDPRSPTMVDEIFGPVLTVYIYEDDQFEETCKLIDQTTTYALTGCIFSDDRAAT
VKAGALLRHAAGNYYINDKSTGAVVGAQPFGGARGSGTNDKAGSMTFFTRWCQPRSVKES
FCPPESFPYPSNQRD