Protein Info for mRNA_915 in Rhodosporidium toruloides IFO0880

Name: 9283
Annotation: K01950 E6.3.5.1, NADSYN1, QNS1, nadE NAD+ synthase (glutamine-hydrolysing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 60% identical to NADE_BOVIN: Glutamine-dependent NAD(+) synthetase (NADSYN1) from Bos taurus

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 70% identity to scm:SCHCODRAFT_67311)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
NAD de novo biosynthesis II (from tryptophan) (7/9 steps found)
NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
NAD salvage pathway V (PNC V cycle) (4/5 steps found)
superpathway of NAD biosynthesis in eukaryotes (10/14 steps found)
NAD de novo biosynthesis I (from aspartate) (4/6 steps found)
NAD de novo biosynthesis IV (anaerobic) (4/6 steps found)
NAD salvage (plants) (6/11 steps found)
aspartate superpathway (15/25 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>mRNA_915 K01950 E6.3.5.1, NADSYN1, QNS1, nadE NAD+ synthase (glutamine-hydrolysing) (Rhodosporidium toruloides IFO0880)
MAASVHPAGGLITVATCSLNQHALDFDGNLERILRSIKIAKEKGAKLRIGPELEITAYGC
QDHFLEGDTQLHSWQVLVKILECEDAYDMVVDVGMPVVHKNVLYNCRIILYQRKILLIRP
KMWLANDGNYRELRWFSPWMKHKQTEDFHLPKMIVDITGQQTVPIGHAVVSTQDTCIGVE
LCEELFTPAAPHIEMALDGVEIFANSSGSHHELRKLRTRIDLIKEATLKSGGLYLYANQQ
GCDGDRLYYDGCAFITLNGRVIAQGSQFSLNDVEVVTATVHLQEIRSHRTWSSRSMQAAG
HEGAFQRVRAETRLSSEDAEDLIETQVDHEGEFEYNVPEEEIAYGPACWLWDYLRRSGMG
GYFVPLSGGIDSCATATIVYSMCRLVAKAAAEGNESVIKDARRIAGEAADSSYVPIDPRE
FCGRIFHTCYMGTSNSSDETRNRAKGLAEAIGAYHTDLNMDACVSAIHTLFAFVTGKKPE
YKVHGGSNAENLALQNIQARLRMLLAYLFAQLLPWVRGKTGALLVLGSANVDEALRGYYT
KYDCSAADINPIGSISKVDLKRFIAWAGKAFELPILDDFLNATPTAELEPITKDYVQSDE
IDMGMTYEELSIYGRLRKQSKMGPYGMFNKLAIEWGHRLSPSDIADKVKYFFRMYAINRH
KMTTLTPSYHAEAYSPEDNRFDLRPFLYNSRWAWQFRAIDAKASAMPDRSKKDKANIGDN
VQAA