Protein Info for mRNA_1089 in Rhodosporidium toruloides IFO0880

Name: 9457
Annotation: K01638 aceB, glcB malate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01274: Malate_synthase
  • amino acids 18 to 531 (514 residues), 711.9 bits, E=2e-218
• TIGR01344: malate synthase A
  • amino acids 30 to 529 (500 residues), 758.2 bits, E=1.6e-232

Best Hits

Swiss-Prot: 62% identical to MASY_EMENI: Malate synthase, glyoxysomal (acuE) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 66% identity to mgl:MGL_1342)

MetaCyc: 58% identical to malate synthase (Saccharomyces cerevisiae)
Malate synthase. [EC: 2.3.3.9]; 3-ethylmalate synthase. [EC: 2.3.3.9, 2.3.3.7]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• chitin deacetylation (3/4 steps found)
• glycolate and glyoxylate degradation II (1/2 steps found)
• butanol and isobutanol biosynthesis (engineered) (5/8 steps found)
• superpathway of glycol metabolism and degradation (2/7 steps found)
• L-arabinose degradation IV (2/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)
• superpathway of pentose and pentitol degradation (6/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.7 or 2.3.3.9

Protein Sequence (546 amino acids)

>mRNA_1089 K01638 aceB, glcB malate synthase (Rhodosporidium toruloides IFO0880)
MQAAKMSPQHNALPQGVQIHGKVEGRHQEILTPAALQFLAVLHRNFNPTRKSLLHRRQLQ
QAQFDAGALPDFLKETEHIRNDPTWQGPAPKPGLEDRRVEITGPVSRKMVIGALNSGAAT
FMADFEDSNSPTWANNLDGQVNLYDAVRRTISFESPEGKKYKLNDKIATLIVRARGWHLD
EAHITVDGQPMSGSMFDFGLFFFHNAKEQLARGFGPYFYLPKMEHYLEARLWNDIFRLSQ
DYVGLPRGTIRATVLIETITASFQMDEIIYELREHSSGLNCGRWDYIFSFIKKMRMHPDF
VLPDRSHVTMTVPFMSSYVKLLIQTCHKRGVAAIGGMAAQIPIKNDEAKNNAVMDKVRAD
KQRECDAGHDGTWVAHPGLVKIAKDIFDKGMVGPNQYHIRREDVTVTALDLLNTNVPGGI
SEDGIRSNCMALLHYCANWVGGLGCVPVANLMEDAATAEISRAQLWHWVKHGASTVDGKK
ITTGLVDKILDEETQKAKKGLDAKKVDLCARYLKSQIRAPALSDFLTTDLTPHLDEYSAP
SQPAKL