Protein Info for mRNA_1457 in Rhodosporidium toruloides IFO0880

Name: 9825
Annotation: K00549 metE 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 319 (316 residues), 365.7 bits, E=2.6e-113
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 7 to 765 (759 residues), 1054.4 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 437 to 760 (324 residues), 477.5 bits, E=2.3e-147

Best Hits

Swiss-Prot: 57% identical to METE_SCHPO: Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (met26) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 67% identity to lbc:LACBIDRAFT_187396)

MetaCyc: 57% identical to cobalamin-independent methionine synthase (Saccharomyces cerevisiae)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (10/15 steps found)
• S-adenosyl-L-methionine salvage I (2/4 steps found)
• seleno-amino acid biosynthesis (plants) (2/5 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (10/18 steps found)
• aspartate superpathway (15/25 steps found)
• superpathway of seleno-compound metabolism (5/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Protein Sequence (767 amino acids)

>mRNA_1457 K00549 metE 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (Rhodosporidium toruloides IFO0880)
MTRSAVLGYPRIGAKREVKKATEAYWAGKISADELQKVAKEQRAERWQTLKAQGVDVIPS
GDFTLYDHVLDANYTFNIIPSRFAKANVSDLDRYFAMGRGRQVGDVDLEAQEMQKWFDSN
YHYIKSEVSSSTEFKLNNLKPVDEFLEAKAAGIVTRPVLVGPITLLLLGKVGRDEPNASS
FEPLSLLDKLVPVYSELLKKLEEAGATEVQIDEPSLVMDVTANLGQQYQSTYEAFARAAP
GLKVTLATYFGRLDKNVEFVAKLPIDSLHVDLDRAPEQLDSVVAAVKPTSLKLSLGLVSG
RNIWKNDFAASLAIANKAIEQLGKDRVVVATSSSLLHTPVTLANETRLKPEERDWFSFAT
EKAAEVATLAKALSGEDVSAALEANKKSIQARRDFEKNSDDAVRERLGKVSQDMYNRKSP
FPARIAAQDKVYNLPTFPTTTIGSFPQTKEIRLARSKHTKGELSTEEYERFLENEIKHAV
EFQEQIDLDLLVHGEPERNDMSQYFGEQLKGFVFTENGWVQSYGSRYVRPPIVVSDVSRP
HPMTVRWSSYAQSLTKRPMKGMLTGPVTILNWSFPRADVPKEVQCKQLALALRDEVVDLE
KAGIFAVQVDEPAIREGLPLRQVDWDAYLTWAVDSFKLATAGVEDRTQIHSHFCYSDFNL
IFEHIKRLDADVISIEASKSDLKLLQAFHRYGYQNLIGPGLYDIHSPRVPSVEEMKERLK
AFKAALPNQKHLFVNPDCGLKTRGWTEVKAALENLVAVAKWARQEYA