Protein Info for mRNA_1675 in Rhodosporidium toruloides IFO0880

Name: 10043
Annotation: K01424 E3.5.1.1, ansA, ansB L-asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR00520: L-asparaginase, type II" amino acids 45 to 379 (335 residues), 375.1 bits, see alignment E=1.5e-116 PF00710: Asparaginase" amino acids 60 to 242 (183 residues), 167.5 bits, see alignment E=2.6e-53 PF17763: Asparaginase_C" amino acids 272 to 377 (106 residues), 68 bits, see alignment E=8.4e-23

Best Hits

Swiss-Prot: 39% identical to ASPG_HELPY: Probable L-asparaginase (ansA) from Helicobacter pylori (strain ATCC 700392 / 26695)

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 51% identity to ani:AN0300.2)

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1

Use Curated BLAST to search for 3.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (393 amino acids)

>mRNA_1675 K01424 E3.5.1.1, ansA, ansB L-asparaginase (Rhodosporidium toruloides IFO0880)
MFVKASLALAAAASLLGTTSAAPLASSFVERSLVERAPVTSVDFNVTYKSNYTEHLPKTL
ILATGGTIAGSSSSATDSTAYTAGVVGIGVLVEAVPQLLNVSNIDGMQVSNVGSESLTDE
IALRLSKLANKALCAADAPYDAVVITHGTDTLEETAFFLDITIGCDKPVVVVGSMRPSTA
ISADGPNNLLQAVTTAVSPNSRARGTLIVLNDRICAAYYCEKTQANTLNTFASPEPGYIG
GLLSDKPFYYMGASKPTFKQVFDISNVTELPHVEIFYSYQGADFELLNTTVANGAKGVVI
AGTGAGSLTDAGMTNVDATVSSGVPVVRSTKINVGFVTPGDYPNTIASGSLNPVKSRRQL
QVLLALNKTNDEIRTAFEEPLKTYLNFNISSYY