Protein Info for mRNA_2060 in Rhodosporidium toruloides IFO0880
Name: 10428
Annotation: K05824 LYS12 homoisocitrate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to LYS12_SCHPO: Homoisocitrate dehydrogenase (lys12) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)
KEGG orthology group: K05824, homoisocitrate dehydrogenase [EC: 1.1.1.87] (inferred from 64% identity to uma:UM04873.1)Predicted SEED Role
"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- butanol and isobutanol biosynthesis (engineered) (5/8 steps found)
- L-lysine biosynthesis IV (5/9 steps found)
- L-lysine biosynthesis V (4/10 steps found)
- coenzyme B biosynthesis (4/16 steps found)
- aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Lysine biosynthesis
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.85
Use Curated BLAST to search for 1.1.1.85 or 1.1.1.87
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (363 amino acids)
>mRNA_2060 K05824 LYS12 homoisocitrate dehydrogenase (Rhodosporidium toruloides IFO0880) MAMQALRESAKTLRIGVIGGDGVGPVVLQAAQRVLEAVPGIPKPSFVPLQAGFEHFQKTG TALPRETVDALKSECTAAMFGAVSSPSHKVAGYSSPIVALRKEMDLYANVRPVKSVEGHD PRVVDMTIVRENTECLYVKQETIEDTPTGRVARATRQISERASTRIGRMAFEIALAAAEV KKQRGGSYGWIGAPTVTIVHKSNVLSVTDGLFRESVRAVAKEYPTVEVNEQIVDSLVYRL FREPEKLSTLVCPNLYGDIVSDGAAALVGSLGLVPSVNVGDSFIMGEPVHGSAPDIADRV PSIANPLASIRSAALMLEFMGYVEPAAKIYDAVDESIRQGKVTPDLGGKLSTEEVTEVVC KQL