Protein Info for mRNA_2092 in Rhodosporidium toruloides IFO0880

Name: 10460
Annotation: K20457 DHFS dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 TIGR01499: bifunctional protein FolC" amino acids 26 to 494 (469 residues), 259 bits, see alignment E=5e-81 PF08245: Mur_ligase_M" amino acids 48 to 302 (255 residues), 32 bits, see alignment E=6.6e-12

Best Hits

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.12, 6.3.2.17

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>mRNA_2092 K20457 DHFS dihydrofolate synthase (Rhodosporidium toruloides IFO0880)
MSSPADQHDLTTTSPSTSMPGISLGLTRIHRLLSLLRSPHLATPVVHIAGTNGKGSVSAY
LSSILSQSKLAVGRFNSPHLVDEWDCIQLHGRPVDEGLFFEAKKEVERVSEREGVGATSF
EVLTATAFSLFARSRPPLDVAVVEVGMGGSEDATNVVPAEKTLLSVVTAVELDHQKFLGD
TVGEIATVKAGITREGGDVVLSVQAHVEATEAVRRVAAERGARVWHAGEAVVIADGEAGA
SRGATAPSAFPPPPLAVIPLEATSVIPPLESHSPSTSASVTAQLPLPGSYQLQNSAAAVL
AAHLLRTLPRTLSLLPSLSQITDDSIRTGVEATRWPGRLSWETYTTSSGRQLPLLLDGAH
NPSSAALLASYLSSLPPALRPTTLVFALSAPRDPADVVRPLLRALAAREAGRRLRVICCG
FSTPVGMEWVRPTAASELAAAVKRIAAEEASRAEVQVEEAPDAEAALRLVENCVDSEGSV
VVAGSLYLVADALRTLRRRQQGGEER