Protein Info for mRNA_2613 in Rhodosporidium toruloides IFO0880

Name: 10981
Annotation: K00223 ERG4 Delta24(24(1))-sterol reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 transmembrane" amino acids 66 to 89 (24 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details amino acids 200 to 221 (22 residues), see Phobius details amino acids 254 to 272 (19 residues), see Phobius details amino acids 278 to 297 (20 residues), see Phobius details amino acids 318 to 341 (24 residues), see Phobius details amino acids 354 to 373 (20 residues), see Phobius details amino acids 434 to 452 (19 residues), see Phobius details PF01222: ERG4_ERG24" amino acids 57 to 495 (439 residues), 370.6 bits, see alignment E=5.3e-115

Best Hits

Swiss-Prot: 43% identical to ERG4_YEAST: Delta(24(24(1)))-sterol reductase (ERG4) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K00223, delta24(24(1))-sterol reductase [EC: 1.3.1.71] (inferred from 43% identity to sce:YGL012W)

MetaCyc: 43% identical to C-24 sterol reductase (Saccharomyces cerevisiae)
Delta(24(24(1)))-sterol reductase. [EC: 1.3.1.71]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.71

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (495 amino acids)

>mRNA_2613 K00223 ERG4 Delta24(24(1))-sterol reductase (Rhodosporidium toruloides IFO0880)
MTDHGRNLRSRTKAAATTSAVPAPASPAKPPAADVAPAQSSSVQPTKEDGGDPHAKEEEL
EFGGPVGTFAMMTGFPILFYYLYVCLYFYDGRLATPKDSSTLLGPGGWVDFVKHVATLVW
ENAMPTKRATALYLIFVGGQFLLAFVMPGVHQKGLPLPHLGNRALDYYCNAYTSIYATAL
LVGLAHYYELFNLAEVIDLYGPLLTVASISGFTLAAITYLFGEKYRMSGNLVYDYFMGAT
LNPRVGIVDIKMFAEIRVSWTLLFALAMGAVVKQYQDYGYVSGNTALFAYGTGLYLNACG
KGEQYIPQTWDMNYEKFGWLLSYWNLAGVPFSYAYAAIYMATHDPATYTYSKPTLVFLFA
LLTFAQAMMDISMAQKSHFKAVKTGTYIKRNTFPQFPWAELDNPKTFETKKGELLLDGCW
KYLRKPNYISDWTQAIIWAASAGTGSVIPYYYPFFHGTMLLHRNARDDVKCARKYGEDWQ
KYKQMVPYSYIPYVF