Protein Info for mRNA_3256 in Rhodosporidium toruloides IFO0880

Name: 11624
Annotation: K01681 ACO, acnA aconitate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01340: aconitate hydratase, mitochondrial
  • amino acids 47 to 778 (732 residues), 1290.9 bits, E=0
• PF00330: Aconitase
  • amino acids 69 to 504 (436 residues), 503.3 bits, E=8e-155
• PF00694: Aconitase_C
  • amino acids 585 to 714 (130 residues), 148.8 bits, E=1.2e-47

Best Hits

Swiss-Prot: 71% identical to ACON_ASPFU: Aconitate hydratase, mitochondrial (acoA) from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 80% identity to scm:SCHCODRAFT_61796)

MetaCyc: 73% identical to aconitase (Aspergillus terreus)
Aconitate hydratase. [EC: 4.2.1.3]

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• TCA cycle III (animals) (10/10 steps found)
• TCA cycle II (plants and fungi) (9/9 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• reductive TCA cycle I (7/11 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• mixed acid fermentation (9/16 steps found)
• reductive TCA cycle II (5/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (783 amino acids)

>mRNA_3256 K01681 ACO, acnA aconitate hydratase (Rhodosporidium toruloides IFO0880)
MVLLSTARKASLRAPTLARSLATPSSANIGQTKVQMSAFDKGHSINYQRIEDNLQVVRSR
LNRPLTLSEKIVYGHLDNPHEADIRPGASYLKLRPDRVACQDATAQMALLQFMSAGLPTV
AVPTTVHCDHLIEAQVGGAKDLARANDINKEVYDFLATATAKYGIGFWKPGSGIIHQIIL
ENYAFPGGLMIGTDSHTPNAGGLGMVAVGVGGADAVDVMADIPWELKCPKRIGVHLTGKI
SGWTTPKDVILKVAGILTVKGGTGAIIEYFGEGVTSLSCTGMATICNMGAEIGATTSVFP
YNERMGQYLDATNRTPIRQYAESFAHNLRPDQNAEYDQVIEINLSELEPHINGPYTPDLA
TPLSKFAQAVKDNNWPDKLSVGLIGSCTNSSYEDMSRAASIADEAASHGLKAQSGFIVTP
GSEQIRATIERDGQMQSLEKAGGVVLANACGPCIGQWDRKDVPKGTKNSIITSYNRNFTG
RNDANTATHAFVASPDLVTAMVFGGSLTFNPMKDSLKGADGQEFKFSDPSGKELPPRGYD
PGENTYQAPPQDRAAVNVAVDPKSDRLQLLEPFKPWDGKDPQGLPVLIKAKGKCTTDHIS
AGGPWLKYRGHLENISNNCLIGAINEANGEANKVQNVFTGEWNTVPETAKEYRDKHHTGW
VVIGDENYGEGSSREHAALEPRFLGGTAVITRSFARIHETNLKKQGMLPLTFKNPQDYEK
VRPDDKVDLIGIKDLKEGSDVTLRVHHKDGKSEDIPLQHTFNAGQITWHRAGSALNHMAK
SKQ