Protein Info for mRNA_3476 in Rhodosporidium toruloides IFO0880

Name: 11844
Annotation: K01915 glnA, GLUL glutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03951: Gln-synt_N
  • amino acids 54 to 102 (49 residues), 41.5 bits, 9.2e-15
• PF00120: Gln-synt_C
  • amino acids 170 to 259 (90 residues), 47 bits, E=1.9e-16

Best Hits

Swiss-Prot: 73% identical to GLNA_HEBCY: Glutamine synthetase (GLN1) from Hebeloma cylindrosporum

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 72% identity to lbc:LACBIDRAFT_183088)

MetaCyc: 69% identical to glutamine synthetase subunit (Saccharomyces cerevisiae)
Glutamate--ammonia ligase. [EC: 6.3.1.2]

Predicted SEED Role

"Glutamine synthetase type II, eukaryotic (EC 6.3.1.2)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Glutamine synthetases or Peptidoglycan Biosynthesis (EC 6.3.1.2)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• ammonia assimilation cycle I (1/2 steps found)
• ammonia assimilation cycle II (1/2 steps found)
• L-aspartate degradation II (aerobic) (1/3 steps found)
• L-aspartate degradation II (anaerobic) (1/3 steps found)
• superpathway of ammonia assimilation (plants) (1/3 steps found)
• L-glutamate and L-glutamine biosynthesis (3/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.2

Protein Sequence (358 amino acids)

>mRNA_3476 K01915 glnA, GLUL glutamine synthetase (Rhodosporidium toruloides IFO0880)
MAHQGLSPYHTHADLLQKYMELPQGGKIQAVWIDAENGLRCKTTTLDGPVSSIDDLKEWN
FDGSSTGQAEGANSDVFLRPAAYFRDPFRRGDNIIVICETYNNDGTPNKYNHRYSCMKTM
EAASKSDPWFGLEQEYTIFGSDGRPYGWPVGGFPGPQGPYYCGVGAGKVFARDMIEAHYR
ACLYAGVNISGINAEVMPSQWEFQVGPCAGKDMGDHLWMARYLLVRIAEEWGVTVSFHPK
PLQGDWNGAGCHTNYSTAETRSDGGMKVIEEMIKKLEKRHMQHIEVYGSDNDLRLTGRHE
TGHIGQFSWGVANRGSSIRVPKSVALAGKGYFEDRRPASNIDPYAVCDIMVQTTLLSA