Protein Info for mRNA_3625 in Rhodosporidium toruloides IFO0880

Name: 11993
Annotation: K01176 AMY, amyA, malS alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 PF00128: Alpha-amylase" amino acids 65 to 401 (337 residues), 80 bits, see alignment E=5e-26 PF02806: Alpha-amylase_C" amino acids 436 to 516 (81 residues), 24.2 bits, see alignment E=8e-09

Best Hits

KEGG orthology group: K01176, alpha-amylase [EC: 3.2.1.1] (inferred from 58% identity to cne:CNG04200)

Predicted SEED Role

"Cytoplasmic alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (528 amino acids)

>mRNA_3625 K01176 AMY, amyA, malS alpha-amylase (Rhodosporidium toruloides IFO0880)
MAPTLPQDPPAPRYRTQNYRGNARGLGKQDHPEGSSNYTLLQGFEWYQEGGGKYYAWLAG
KAKELGEMGITAIWIPPPTKADGPNSVGYSIYDLWDLGEFDQKGGRATKWGTKEELLECV
RKLKENGIVVYIDAVLNHKAGADYTEPFHATEVDWDDRTKEISDLYEIEGWTGFNFPGRK
GKYSEMIWSHIHFTGVDWDDKGKKKAIFRIQGEGKTWAKSVDKEKGNFDYLLGSDIDHSH
PEVRKDLIDWGEWVIKETGAAGFRFDAVKHIDEGFIADFVREVRERLDNPDMFCVGEFWK
SDLGALEGYLDRFPEQFSCFDVTLHDNFHRAGTEAESFDLRSILDNSLVRSRPMDAVTVV
CNHDTQPTQALEAPVAPWFAPLAYCLTLLRAEGYPCVFGGDLWGCKSEPAVEPISGLAEL
VKARRWFAYGPTRDYWDHANCLGWIREGDADHDGCAVVICNGTGEGEKRMQVQGNNEHKG
EKWVDVLGWTQGEVEIEEDGWATFRCPSKSVAVWVKKDARGLEAFGKK