Protein Info for mRNA_3654 in Rhodosporidium toruloides IFO0880

Name: 12022
Annotation: K11204 GCLC glutamate--cysteine ligase catalytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03074: GCS
  • amino acids 7 to 347 (341 residues), 362.2 bits, E=2.1e-112

Predicted SEED Role

"Glutamate--cysteine ligase eukaryotic (EC 6.3.2.2)" (EC 6.3.2.2)

MetaCyc Pathways

• γ-glutamyl cycle (5/6 steps found)
• glutathione biosynthesis (2/2 steps found)
• ophthalmate biosynthesis (2/3 steps found)
• homoglutathione biosynthesis (1/2 steps found)
• ergothioneine biosynthesis I (bacteria) (3/7 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.2

Use Curated BLAST to search for 6.3.2.2

Protein Sequence (406 amino acids)

>mRNA_3654 K11204 GCLC glutamate--cysteine ligase catalytic subunit (Rhodosporidium toruloides IFO0880)
MSPPPAQRFHIPKSRYGYDSVDCYIADTPFNRPKYNDNNMPIDPKIRDHLIKRGVDPLLA
THFAHLFIWDPIVIFEETMCQDNAKSSDHFESIQSTNWRTVRFKLPPPGSPIGWRVEFRS
MEVQLTNYENAAFSVFIVLLTRAIMMLGLNFYLPISKVDENMHRAHRRNAINTQNFYFRK
NPVPSTSDDATLPSASAFGRGRTNGFAAHVRDASTASSDYPASPTVCSSHAPSRSASPTS
DIAFGPVEDEYDEFSINEIINGSKEKGFPGLIGVVERYLDGLEKEGKVSEEVRKGIDRSL
ELIRRRADGSLITLATYIRRFVRSHPSYAHDSVVSQEINYDLIRTLDEDKKGKGAFVRCC
GDVVGDVAADDAQSEGSEGLSAGAAVIEDKGLCCGPMEPASAAKEA