Protein Info for mRNA_4226 in Rhodosporidium toruloides IFO0880

Name: 12594
Annotation: K00700 GBE1, glgB 1,4-alpha-glucan branching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 PF02922: CBM_48" amino acids 68 to 152 (85 residues), 68.6 bits, see alignment E=7.2e-23 PF00128: Alpha-amylase" amino acids 219 to 306 (88 residues), 50.8 bits, see alignment E=3e-17 PF02806: Alpha-amylase_C" amino acids 599 to 692 (94 residues), 93.8 bits, see alignment E=1.1e-30

Best Hits

Swiss-Prot: 69% identical to GLGB_RHIID: 1,4-alpha-glucan-branching enzyme (GLC3) from Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 72% identity to ppl:POSPLDRAFT_110441)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (693 amino acids)

>mRNA_4226 K00700 GBE1, glgB 1,4-alpha-glucan branching enzyme (Rhodosporidium toruloides IFO0880)
MPPVSTNPPDVLKQPKKQSEFAVIREDEYLEPFEPAIKARTAKFNSWVETLNAHEGGLER
FSKGYESYGLHALPNGDVKYREWAPNATSACLIGDFNGWDRAANPMTKNDFGVWECVVKS
KGGQAAIPHNSKVKISMTTPSGERIERLPAWATRVVQDLTVSPIYDAVFWNPPKNERYTF
KNSRPKQAPRAPKVYEAHVGISTPEPKIGSYKEFTRDVLPRIKDLGYNTIQLMAIMEHAY
YASFGYQITSFFAISSRYGTPEELKELIDTAHGLGLTVLLDVVHSHASNNSADGLNHFDG
SDHQYFHGGGKGRHELWDSRLFNYGHHEVLRFLLSNLRFYMEEYQFDGFRFDGVTSMLYV
HHGIGTGFSGGYHEYFGDKVDEEAVVYLMLANTMLHELYPTCLTIAEDVSGMPALCRTVA
EGGIGFDYRLAMAVPDMWIKLLKEAPSDESWDMGNIAFTLTNRRWKEKSIAYAESHDQAL
VGDKTLAFWLMDKEMYTNMSDLTERTPVIDRGLALHKMIRLITHALGGEGYLNFEGNEFG
HPEWLDFPREGNGDSYQYARRQFNLIDDHLLRYKYLYAFDRAFNRLEDQYQWLSSPPAYV
SLKNESDKVIVFERGNLLWIFNFHPTNSFTDYRVGTDWAGEYQVVLSSDDPEFGGHNRVD
KSVKHFTTPMEWNGRKNWLQVYLPNRTVQVLAH