Protein Info for mRNA_4257 in Rhodosporidium toruloides IFO0880

Name: 12625
Annotation: K00295 MTD1 methylenetetrahydrofolate dehydrogenase (NAD+)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 21 to 139 (119 residues), 95.4 bits, E=3e-31
• PF02882: THF_DHG_CYH_C
  • amino acids 201 to 264 (64 residues), 30 bits, E=3e-11

Best Hits

KEGG orthology group: K00295, methylenetetrahydrofolate dehydrogenase (NAD+) [EC: 1.5.1.15] (inferred from 54% identity to cne:CNF03710)

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• formaldehyde oxidation VII (THF pathway) (3/4 steps found)
• folate transformations II (plants) (8/11 steps found)
• folate transformations I (9/13 steps found)
• reductive glycine pathway (6/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (8/12 steps found)
• purine nucleobases degradation II (anaerobic) (14/24 steps found)
• L-histidine degradation III (1/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (26/57 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.5, 3.5.4.9

Use Curated BLAST to search for 1.5.1.15 or 1.5.1.5 or 3.5.4.9

Protein Sequence (387 amino acids)

>mRNA_4257 K00295 MTD1 methylenetetrahydrofolate dehydrogenase (NAD+) (Rhodosporidium toruloides IFO0880)
MSAPAPKPPADPTAISCVTVLGEHIAKPFRTAIQEATSKLAQKPKLVGILANSGAPSRAY
ANWTAKACEAVGIEYELREVGIVAEGATEGTAEQGEVEEAILAANEDDGVNGIMVYYPIF
GPRQDGYLQQAVSPLKDVEGLNFTWLFSLYHNIRFIDPRKLALAAQVLTPSPLPASASST
VPTTSTSTPSHSSTTDDGLVKAILPCTPLAIVKTLEHCQVYNPLRPYGSRAFGRTITVVN
RSEVVGRPLAALLSNDGARVFSVDLDGIQEFTRRKLVEGEEGAKASFHPYHVVKKCELSF
EECLKASDVVIGGVPSKSYKIPTSALKEGVVAINFSESKNFEDDIKEKASIYCPGIGKAT
IVLLQRNLLRLREYQASLRGEVKGEKQ