Protein Info for mRNA_4316 in Rhodosporidium toruloides IFO0880

Name: 12684
Annotation: K03178 UBE1, UBA1 ubiquitin-activating enzyme E1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1023 transmembrane" amino acids 856 to 876 (21 residues), see Phobius details amino acids 896 to 912 (17 residues), see Phobius details amino acids 949 to 966 (18 residues), see Phobius details TIGR01408: ubiquitin-activating enzyme E1" amino acids 15 to 1021 (1007 residues), 1305.6 bits, see alignment E=0 PF00899: ThiF" amino acids 20 to 399 (380 residues), 101 bits, see alignment E=1.9e-32 amino acids 415 to 916 (502 residues), 229.8 bits, see alignment E=8.4e-72 PF16190: E1_FCCH" amino acids 192 to 263 (72 residues), 94.8 bits, see alignment 7.2e-31 PF16191: E1_4HB" amino acids 264 to 331 (68 residues), 92.1 bits, see alignment 5.1e-30 PF10585: UBA_e1_thiolCys" amino acids 603 to 850 (248 residues), 297.2 bits, see alignment E=3.7e-92 PF09358: E1_UFD" amino acids 925 to 1017 (93 residues), 123.3 bits, see alignment E=1.4e-39

Best Hits

Swiss-Prot: 56% identical to UBA1_SCHPO: Ubiquitin-activating enzyme E1 1 (ptr3) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K03178, ubiquitin-activating enzyme E1 [EC: 6.3.2.19] (inferred from 63% identity to cci:CC1G_14580)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.19

Use Curated BLAST to search for 6.3.2.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1023 amino acids)

>mRNA_4316 K03178 UBE1, UBA1 ubiquitin-activating enzyme E1 (Rhodosporidium toruloides IFO0880)
MAAPAQQPVSNDEGLDESLYSRQLYVLGHEAMKRMAASDVLVIGLSGLGVEIAKNICLAG
VKSVTLSDPTPVSLPDLGTQFFLRDSDVGQRRDEATRPRLAELNAYTPVSVLPDLNLDAV
KRFTVVVLVQGSTEDQLKLNDFTHANDIKFIAAETAGLFASAFCDFGEQFPVVDQTGENP
LSGMVVEIEEAEDALVTCLDESRHGLEDGDYVRFSEVEGMNINSDGVEGARKITVTGPYT
FKIGDTRGLGQYKRGGWFHQVKMPKMYDFKPLRESMTAPEFLITDFAKFDRPPTLHIGFQ
ALSAYRTQHNRLPKPRNPADAATFVQLAKDIAAQVKFEGDLDEKVLEELAFQAVGDLAPV
NAVIGGFVAQEVLKACSGKFSPLYQHLYFDALEALPVDLPFEEECAPSNPASRWDNQIAV
FGRSFQQKIENNRQFLVGSGAIGCEMLKNWAMMGVANGQNGHVYITDLDTIEKSNLNRQF
LFRPKDVGSFKSEAARRAATEMNAGLDGKVEVFKLAVGPDTENVFGDSFFDSIDCVTNAL
DNVSARQYMDQRCVYYAKPLLESGTLGTKGNVQVVLPHVTESYSSSQDPPEKSHPSCTIK
NFPNQIEHTIAWAKERFEEFFEKPAEVVNSYLSQPNFVEAAKQSGETAQLLKVKSYLVDH
KPLGFEECITWARQQFETEYVNEIKQLLFSLPKDLLTKEGTPFWSGPKRAPDPLQFDYNN
PTHMEFIVAAANLHAYNYGLKGETNLDTFKRVLDQVHLPEFVPKSGVQVQIKDDEPVNND
KDAPPSTDADDLTSLAASLPPPSTLAGYRMVPAQFEKDDDTNHHIDFITAASNLRAQNYG
IQPADRHRTKGIAGKIIPALATTTAVVAGLVCIELYKLLDEKKRGADVLERYKNSFCNLA
LPFFAFSEPIAAPKMKYRDTEYTLWDRFEIEGDITLEQLVEHFKEKYKLEISMLSSGVSM
LYSGFMPKKKLEERLKMPLSQLVETVSKKPIPEHVRDIVFEIMCNDMDTDDDVEVPYLKV
RVR