Protein Info for mRNA_4374 in Rhodosporidium toruloides IFO0880

Name: 12742
Annotation: K00232 E1.3.3.6, ACOX1, ACOX3 acyl-CoA oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14749: Acyl-CoA_ox_N
  • amino acids 40 to 152 (113 residues), 93.9 bits, E=2.3e-30
• PF02770: Acyl-CoA_dh_M
  • amino acids 154 to 270 (117 residues), 45 bits, E=2.1e-15
• PF01756: ACOX
  • amino acids 515 to 678 (164 residues), 148.7 bits, E=2.8e-47

Predicted SEED Role

"Peroxisomal acyl-coenzyme A oxidase (EC 1.3.3.6)" (EC 1.3.3.6)

MetaCyc Pathways

• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• fatty acid β-oxidation II (plant peroxisome) (4/5 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• methyl ketone biosynthesis (engineered) (4/6 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (2/5 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (3/10 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (4/13 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (3/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (3/12 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (4/14 steps found)
• jasmonic acid biosynthesis (7/19 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Fatty acid metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.6

Use Curated BLAST to search for 1.3.3.6

Protein Sequence (702 amino acids)

>mRNA_4374 K00232 E1.3.3.6, ACOX1, ACOX3 acyl-CoA oxidase (Rhodosporidium toruloides IFO0880)
MSYQPPSSVPVEIPDGFKPLGTSGSRLLEIERSKASFSPADLEKYIYGDAAIERRNRILP
VVENEPAFDKSQIHYMDRGAKYRHGLAKEKRLVQLTRELGWTAEDVRTAEELLDMPAAFG
LHNSMFLKTLRAQSTDEQRELFLKPAENYEIIGCYAQTELGHGSNVQGLETTAVYKPESK
SFVINTPGMSSMKWWIGGLGRTADHAVVMAQLYTPDGKNGSLVRRGPYPFVVPLRDTKTR
ELLPGRTIMDIGPKAGYPMTDNGTAIFNNVEIPHVNFLAKFASVDVESGRFSKPPHDKLA
YGTMIYIRAGIVQQARMILARSATVAIRYCSVRRQFADRDAPVTDDGRKPAETPVVNYQL
VQARIFPPLVQAFACHYTGREMMRQYESNESAMAEGDFSLLADLHATSSGLKSLCTLMAS
NAIEECRRACGGHGYSLSSGLASLWSDYLPQVTWEGDSYMLTQQTGRYLFKTFRTILSDP
NAEMSRENLTAHYVRKYIANPEAKAPFKYAGDLSDPQLFIDAFGHRAAYLTATALRKRDI
EKRTWNSLLIDIFRCSTAHAQYYLVYNFAQAIMHDQELKAQPALHRIMTTCFELFACYTM
DAEASEFLSSRYLSPQQHELLRNRVHALLAELRPQAVPLVDSWNIPDYLLNSALGRKDGD
VYPALVRFAQGEPLNQTRFNVDVNDEQLEVGKEEGVRAWSKL