Protein Info for mRNA_4599 in Rhodosporidium toruloides IFO0880

Name: 12967
Annotation: K12820 DHX15, PRP43 pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 825 transmembrane" amino acids 763 to 781 (19 residues), see Phobius details PF00270: DEAD" amino acids 96 to 244 (149 residues), 32.1 bits, see alignment E=2.5e-11 PF00271: Helicase_C" amino acids 288 to 424 (137 residues), 45.2 bits, see alignment E=2.8e-15 PF04408: HA2" amino acids 487 to 575 (89 residues), 81.1 bits, see alignment E=1.8e-26 PF07717: OB_NTP_bind" amino acids 636 to 711 (76 residues), 60.7 bits, see alignment E=3.6e-20

Best Hits

Swiss-Prot: 66% identical to PRP43_YEAST: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (PRP43) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K12820, pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43 [EC: 3.6.4.13] (inferred from 69% identity to cnb:CNBF0920)

Predicted SEED Role

"RNA helicase, putative"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (825 amino acids)

>mRNA_4599 K12820 DHX15, PRP43 pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43 (Rhodosporidium toruloides IFO0880)
MAEPSSKRARTDAPVENPYLAHLNNPADRMTGGANGGKGGFEGWIPRKQGGKEVEAVMAG
DFNPFNQRPFSNRYREIFEVRKKLPVHQQMDEFLKMFNANQFVVLSGETGSGKTTQIPQY
VAYADLPHLRKPGMQVACTQPRRVAAMSVAKRVADEMDVSLGEEVGYSIRFEDCTSPNTF
LKYMTDGMLLREAMNDHMLSRYSCIILDEAHERTLATDILMGLLKDVAKRRPDLKIVVMS
ATLDAAKFQNYFFGAPLLKVPGRTFAVEIFYTPEPEPDYLEAAIRTVLMIHQAEPEGDIL
VFLTGEEEIEDACRKIMVEADQLPHQFGPLKAIPLYSSLPPHQQQRIFDKAPPPRTPDGP
PGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKIYNPRIRVESLLVSPISKASANQRAGR
AGRTRPGKCFRLYTESDFVKELEEQTYPEILRCNLASVILELKKLGIDDLVHFDYMDPPA
PETVMRALELLNYLAAFDDDGNLTPLGDLMAAFPLEPQLAKMVIVSPEFKCSNEILSIAA
MLSVPNPYLRPNSQHKEADEAKAQFAHPAGDHLSLLNLYHAYKSNPDANWCWNNYVSFRA
MQQADNVRNQLKRTMEKLDLDLISTSFEDKSYYENIQRALTAGFFMQVAHNEGDKKGYVT
IKDLQVVGLHPSTGLDHKPEWVIYNEFVLTTRNFVRTVTETRPEWLWEYGQLYFDPTNDR
SFPDCEARRVFERMQKGQYRPSKRSGNDADKRDKKKRKERRSLVGSRAVILCHFVTPALL
PGRVAFSRCYSFASQAALGTVSEPEAAGVCKHGRKRGERNSRSSK