Protein Info for mRNA_4649 in Rhodosporidium toruloides IFO0880

Name: 13017
Annotation: K03934 NDUFS1 NADH dehydrogenase (ubiquinone) Fe-S protein 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF13510: Fer2_4" amino acids 32 to 109 (78 residues), 62.4 bits, see alignment E=8.4e-21 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 36 to 631 (596 residues), 716 bits, see alignment E=2.4e-219 PF00111: Fer2" amino acids 37 to 89 (53 residues), 32 bits, see alignment 2.5e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 116 to 155 (40 residues), 62 bits, see alignment 7.6e-21 PF00384: Molybdopterin" amino acids 306 to 633 (328 residues), 220.3 bits, see alignment E=1.2e-68 PF09326: NADH_dhqG_C" amino acids 682 to 721 (40 residues), 31.4 bits, see alignment 6.1e-11

Best Hits

Swiss-Prot: 65% identical to NDUS1_NEUCR: NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial (nuo78) from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

KEGG orthology group: K03934, NADH dehydrogenase (ubiquinone) Fe-S protein 1 [EC: 1.6.5.3 1.6.99.3] (inferred from 71% identity to scm:SCHCODRAFT_68699)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3, 1.6.99.3

Use Curated BLAST to search for 1.6.5.3 or 1.6.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (752 amino acids)

>mRNA_4649 K03934 NDUFS1 NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Rhodosporidium toruloides IFO0880)
MLRLSRRIPASSRNPLALASSRAIHATSRASADVTLEIDGVPVTVPQGTALIQACEKAGA
TIPRFCYHDRLNIAGNCRMCLVEVSPGPPKPQASCALPAMPGQKVKTNSPTVHKAREGVM
EFLLANHPLDCPICDQGGECDLQDQSMRYGSDRTRFHEITGKRATEDKNFGPLIKTSMNR
CIHCTRCVRFANEVAGVQDLGTSGRGNDMQIGTYIEKTINSEMSGNVIDLCPVGALTSKP
YAFEARPWELKKTESVDVLDAVGSNIRVDSRGLVVMRVEPRLNDDINEEWISDKTRFAYD
GLRTQRLNTPLVRVGDRFQPVTWQNALGAIKDGLASSGAKGNEIQAVAGSLADSESLVAL
KDLVNRLGSDNTALDQPLGQAPPAHGVDHRAAYTFGSSIAGVEYADRVLLIGTNPRHEAA
ILNARLRKTWLRNETDCALIGEQFDSTFGYDHLGADANAVRDVLSGKKGDWAKQLKEAKR
PMIIVGSAVTEHEEGAKVFAEVAKFVEANKDKFVTPEWTGYNVLQRAASRTAAYDIGFVP
SAAAGKTTPKFVYLLNADDFSPSSIPRDAFVVYQGHHGDVGAQYADVVLPGCAYTEKSVT
WVNTEGRTQLGRAAVPPPGAAREDWKIIRALSEVLGETLPYDDTGALRDRMWDVSPTLVR
YGELEKPSSVLEAVQALRKQGEREAGKRNGLGGRKGEFRKVFDSFYRTDPVSRNSVTMGQ
CVKTFEQGIDGVEKPVIGDDGMAQAGHHPARA