Protein Info for mRNA_4901 in Rhodosporidium toruloides IFO0880

Name: 13269
Annotation: K03163 TOP1 DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 845 PF02919: Topoisom_I_N" amino acids 227 to 441 (215 residues), 328.9 bits, see alignment E=2e-102 PF01028: Topoisom_I" amino acids 445 to 670 (226 residues), 328.7 bits, see alignment E=2.5e-102 PF14370: Topo_C_assoc" amino acids 776 to 844 (69 residues), 103.7 bits, see alignment E=5.8e-34

Best Hits

KEGG orthology group: K03163, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 57% identity to cnb:CNBH3140)

Predicted SEED Role

"DNA topoisomerase I, eukaryotic-type (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (845 amino acids)

>mRNA_4901 K03163 TOP1 DNA topoisomerase I (Rhodosporidium toruloides IFO0880)
MNDATAEALDPKAKQLAVFTTGESDSEDDVPLAQQAAAAGVAVTDGSGANGKKRAASESD
SEDEKPLAQTAAGKKRSNGRPASKDYSSSEDEKPLAKTAAKSKRPRASTSRAKSMKEESE
DEEDFSETEDASDDYSSSSDTPLAKKKKAPAKGKGKAKAAPAKKATPAKKAAPKKPKKEE
DEGSASPAPRKGKGKAKKEDDEESREAGSGDEDVYKWWENQNDGEEKWKTLEHNGVLFPP
EYTPHGVKMNYDGKPVELAPEAEEVASFFAAILETDYVKNPVFVRNFFDDWQKVLKAHPC
PDGTKIKSYEKCDFKPIFAYLEAEKQKKKSMTAAEKKAAKAEKDALEEKFKWCYLDGRKE
KVGNFRIEPPGLFRGRGEHPKTGMLKTRVRPEQITINIGKGVEVPPPPPGHKWHKVVHDN
KVTWLATWKENINGNVKYVFLAAGSSLKGQSDLKKFEKARSLKEHVDRIRADYTADLKSK
EMATRQRATAIYLIDRFALRAGNEKGEDEADTVGCCSLRFEHVTLTPPNKVTFDFLGKDS
IRYVNEVEVDEQVFKNFKIFKKEPKTVGDLLFDRLNTSVVNKYLTSYMDGLTAKVFRTYN
ASWTFAQQLKNTPADASVADKLLAYNRANRLVAVLCNHQKSVSKGHSAAMEKMHDKIRAL
KYQRMKLRKQLFANADKPKKHKQAYGDDESDLDDDWIQEHEKALVVQERDKIRKKFDKEN
SKREADGEKIMPEKELNERLKAADALEKKIKAERKAGYEESSQSEDKILKALEKIDDRIK
VAKTNFVDKDEGKEISLGTSKINYIDPRISVAWCKKFDVPLNKVLTKTLLEKFTWATHVE
ADFEW