Protein Info for mRNA_5075 in Rhodosporidium toruloides IFO0880

Name: 13443
Annotation: K00958 sat, met3 sulfate adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14306: PUA_2
  • amino acids 4 to 165 (162 residues), 184.5 bits, E=1.7e-58
• TIGR00339: sulfate adenylyltransferase
  • amino acids 5 to 388 (384 residues), 508.8 bits, E=8.1e-157
• PF01747: ATP-sulfurylase
  • amino acids 174 to 388 (215 residues), 292.1 bits, E=3.5e-91
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 393 to 567 (175 residues), 143.3 bits, E=6.9e-46
• PF01583: APS_kinase
  • amino acids 396 to 558 (163 residues), 172.2 bits, E=1.2e-54

Best Hits

Swiss-Prot: 70% identical to MET3_USTMA: Sulfate adenylyltransferase (MET3) from Ustilago maydis (strain 521 / FGSC 9021)

KEGG orthology group: K00958, sulfate adenylyltransferase [EC: 2.7.7.4] (inferred from 71% identity to lbc:LACBIDRAFT_183631)

Predicted SEED Role

"Sulfate adenylyltransferase (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (7/9 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• sulfite oxidation III (1/2 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/4 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/5 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/5 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/11 steps found)
• superpathway of seleno-compound metabolism (5/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.25

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Protein Sequence (579 amino acids)

>mRNA_5075 K00958 sat, met3 sulfate adenylyltransferase (Rhodosporidium toruloides IFO0880)
MANSPHGGVLKDLHARDAPIRQQLIQESETLPDIRLTERQLCDLELIMNGGFSPLEGFMT
KEDYESVVETLRLTDGALFSMPITLDVSKEQIDSLKLTPGSRVTLRDSRDESPLAILSIS
SIYQPNKSNEAEKVFGADDQAHPAVHYLHNSVKDFYVGGAVQAINSPEHYDYVELRYTPR
ELRAYFEKLSWTRVVAFQTRNPMHRAHRELTVRAARQQRANVLVHPVVGLTKPGDVDHFT
RVRVYEAIIRTYPKGMAALALLPLAMRMGGPREAVWHAIIRKNFGATHFIVGRDHAGPGK
NSQGKDFYGPYDAQELVTKFKDELQIEMVPFQMMSYIPDQDTYMPVDEIPKGTVTADISG
TELRRRLKTGAPIPDWFSYEAVVKVLRESYPPKARQGFTILLTGYVNSGANAIAKALESQ
FQQQAGRSVSLLLGDTIRSELSAELGFAPEERHKNLQRLAFVAAELSRAGAAVIATPIAP
YDHSRKAAKAHIVQNGGAGGGNFFLVHVATPLEHCEKTDRRGVYAKARSGEIKGFTGIDD
KYDAPTDADLVVDLSKQSVSSATHAIILLLESNGLIGSA