Protein Info for mRNA_5078 in Rhodosporidium toruloides IFO0880

Name: 13446
Annotation: K01870 IARS, ileS isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1117 TIGR00392: isoleucine--tRNA ligase" amino acids 20 to 903 (884 residues), 903.7 bits, see alignment E=6.6e-276 PF00133: tRNA-synt_1" amino acids 31 to 673 (643 residues), 698.3 bits, see alignment E=1.2e-213 PF08264: Anticodon_1" amino acids 731 to 888 (158 residues), 86.6 bits, see alignment E=2.7e-28

Best Hits

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.5

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1117 amino acids)

>mRNA_5078 K01870 IARS, ileS isoleucyl-tRNA synthetase (Rhodosporidium toruloides IFO0880)
MTTPQPTNPNAQFPPHDPTAQFSFPKAEEEVVEYWRAIDAFRTSVQQSKDEGRPNYVFLD
GPPFATGLPHHGHLLMGTVKDIVTRFWHSNGFHVERRFGWDTHGLPVEHEIDKKLGITSK
ADVMAMGIDKYNAECRAIVMRYASEWRRTVERLGRWIDFDNDYKTLYPTFMESVWWVFGQ
LWKKNLVYRGLKVMPYTTGCTTPLSNFEAGQAYKDVQDPAVTVAFTLVDDPKTSFLAWTT
TPWTLPSNLGLCVHPDFTYVKIHDVERDADFILCDKLLNALYKDPAKAKKEKKFEVLETY
KGSDLVDIKYEPLFPYFVEKYKSRAYRVLSDPYVTDSSGTGIVHQAPAFGDDDHRVAVAH
GVVEKDEMPPCPIDEKGVYTDEVPDFKGMYVKDADKHITKFLKNAGKIIVASTLTHSYPF
CWRSGTPLIYRAIPVWFVRVQGHQDELVKNNKATRWVPASVGENRFQNWLENARDWNVSR
NRYWGTPLPLWASEDYEEIVCISSIAELEELSGVKGITDLHRDKIDHITIPSKQGKGQLK
RVEEVFDCWFESGSQSVELGDRRTDNDSTYSMPYAQVHYPFENKDAFEHSFPADFISEAM
DQTRGWFYTLLVLSNHLFGTAPWKNLIVCGHVLAADGKKMSKSLKNYPDPNMIIDRYGAD
ALRLFLINSPVVRGDSLRFKEEGVRDVIGHVLLPLLNSFRFFLGQAALLKKEQGVEFKYD
PTSLGSSNVMDRWILARCQTLIKLVRTEMEAYRLYTVVNQMLGLIDELTNWYIRFNRRRL
KGENGLEDTTHALNTLFETLFTLVRTLSSFTPFLTEHIYQGLRPFFPHDGESLGCGADLR
SLHFLSFPKAREEYFDDVIERQVSRLQAVIELGREVRQRMVIPVKTPLAELVVFHPEQQY
LDDVKSLLPYVEQELNVRTVVFTSDEARCGIKFRADADYAVLGRKLRKDLARVKKALPSV
PSDDVKAYLSTGKITVDGVELVKGDLTAMRYVELPPRVEGGPQYETNTNNDVVILLDCLR
RPELEQEGTAREVINRVQRLRKKAGLVATDDIDVFYSFADGVGKELEEIMAASGDVIRKV
LKKSPRPKSEFKGDKEVIIEEEQEISDDKVVLTLVWA