Protein Info for mRNA_5354 in Rhodosporidium toruloides IFO0880

Name: 13722
Annotation: KOG0557 Dihydrolipoamide acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF00364: Biotin_lipoyl" amino acids 31 to 103 (73 residues), 62.6 bits, see alignment E=2.5e-21 PF02817: E3_binding" amino acids 172 to 205 (34 residues), 30.3 bits, see alignment 4.3e-11

Best Hits

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.12

Use Curated BLAST to search for 2.3.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>mRNA_5354 KOG0557 Dihydrolipoamide acetyltransferase (Rhodosporidium toruloides IFO0880)
MLAVLRTARQATRPLARQLHASSAVAAYSNFLMPAMSPTMTEGSISEWKVKEGEAYTAGA
VLLSIETDKATIDVEAQDDGVMGKILVGDGAAGVQVGKLIAILAEEGDDLANIEIPSEEA
AASSSAPPPSDSKSEAGPTSPTASDAPTPSPATPSPTPSATHAHPHHSKPLLPSVLRLLA
LNGIQDASEIKGTGHNGVLTKGDVLAFMGKISSPRGSLPKEKPHGGHPANEYTATPKDVK
KPVEVQLDGPSMRRIIASGLATAAAGPKPAIVSAPVFSFDSILDDYLPPSQRSSARSASS
ASPVPPPPAANQNAFDAILGL