Protein Info for mRNA_5372 in Rhodosporidium toruloides IFO0880

Name: 13740
Annotation: K04487 iscS, NFS1 cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 68% identity to cne:CND05560)

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7)" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

L-alanine biosynthesis III (1/1 steps found)
superpathway of L-alanine biosynthesis (3/4 steps found)
cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
bis(guanylyl molybdopterin) cofactor sulfurylation (2/4 steps found)
thiazole component of thiamine diphosphate biosynthesis II (4/7 steps found)
tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
thiazole component of thiamine diphosphate biosynthesis I (3/7 steps found)
tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
superpathway of thiamine diphosphate biosynthesis II (5/11 steps found)
molybdopterin biosynthesis (1/6 steps found)
superpathway of thiamine diphosphate biosynthesis I (4/11 steps found)
tRNA-uridine 2-thiolation (cytoplasmic) (1/7 steps found)
[2Fe-2S] iron-sulfur cluster biosynthesis (2/10 steps found)
tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>mRNA_5372 K04487 iscS, NFS1 cysteine desulfurase (Rhodosporidium toruloides IFO0880)
MHRATAAITRTARQAGNKAVASAATPRRTLVSSSSLGSIYAASATAGTSARRVSSSSSTS
SDACRRSLATVATNIPLGNTTSIPIEVESEPATVVTPTSDMAGFEITAGESSQVTGKSRE
DLGRPIYLDAQATTPVDPRVLDAMLPYFTNEYGNAGSKTHAYGWESEKAVELARKQVADI
IGADPKDIIFTSGATESNNMAIKGIARFYKGKRRHIITTQTEHKCVLDSCRVLQDEGFDV
TYLPVQANGVVDLKLLEESIRPDTVLVSIMMVNNEIGTMQPIKEIGEIVKKHKGVFFHTD
AAQAIGKVPVNVDDLKVDVMSMSGHKIYGPKGVGCLYVRRKPRVRLEPIINGGGQERGLR
SGTVPVPLVVGLGEACRLAKKEMAADSAHIRRLAERLYNGITAELTHVVRNGDPDGYPGC
TNLSFAAVEGESLLMSIKDIAVSSGSACTSASLEPSYVLRALGAEEDMAHSSIRFGIGRF
TTEEEVDYVVEKVIKSVQRLRDMSPIWDMLMEGIDISQVDWTQH