Protein Info for mRNA_5445 in Rhodosporidium toruloides IFO0880

Name: 13813
Annotation: K07513 ACAA1 acetyl-CoA acyltransferase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 transmembrane" amino acids 399 to 419 (21 residues), see Phobius details PF00108: Thiolase_N" amino acids 35 to 290 (256 residues), 274.7 bits, see alignment E=8e-86 TIGR01930: acetyl-CoA C-acyltransferase" amino acids 37 to 418 (382 residues), 416 bits, see alignment E=7.3e-129 PF02803: Thiolase_C" amino acids 299 to 419 (121 residues), 143.6 bits, see alignment E=2.4e-46

Best Hits

Swiss-Prot: 54% identical to THIKB_RAT: 3-ketoacyl-CoA thiolase B, peroxisomal (Acaa1b) from Rattus norvegicus

KEGG orthology group: K00632, acetyl-CoA acyltransferase [EC: 2.3.1.16] (inferred from 68% identity to cne:CNA04700)

MetaCyc: 53% identical to 3-ketoacyl-CoA thiolase, peroxisomal (Homo sapiens)
RXN0-6512 [EC: 2.3.1.223]; Acetyl-CoA C-acyltransferase. [EC: 2.3.1.223, 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.223, 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.223, 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.223, 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.223, 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.223, 2.3.1.16]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)" (EC 2.3.1.16, EC 2.3.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.223 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>mRNA_5445 K07513 ACAA1 acetyl-CoA acyltransferase 1 (Rhodosporidium toruloides IFO0880)
MSSTISAAKERLSGLVSHLTPRGKAALTQQNDDDVVIVSAVRTPITRAKKGGFKDACPED
LLKAVFEAAVDRAGIKKELVEEIQVGNVLPAGGGATVARMAQLAAGFPTTSSIATVNRQC
SSGLVAVNHIALMIASGQIDVGIGAGVESMTQGYGAGAMPEKMSDDILECQQAADCLLPM
GITSENVATEYKVSREAQDEFAAESFRRASAAQKAGKFKDEIVPVKVKWTDPKTEEEKEI
TVSQDDGIREGVTKESLSKLKPVFSKTGSTHAGNASQVSDGAAAVLLTRRSKAKELGLPI
LAKFACAAITGVEPKLMGIGPAFAIPKVLEKAGISKNEVDLYELNEAFASQAVMSIEHIG
LDRSKVNPNGGAIAMGHPLGCTGARQIATALAESKRSGARIICTSMCIGSGMGMASIIVA
EN